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 | | The MHC tetramer assay (Altman et al., 1996) (and a related method using MHC-Ig chimeras (Greten et al., 1998; Hamad et al., 1998)) was developed in response to this need. | | | In the remainder of this review, we will focus on features of MHC tetramer analysis and results that are most relevant to the study of immune responses to HIV, and to the development of an HIV vaccine. | | | This points to one of the limitations of the tetramer technique-tetramer staining provides precise measurements of a very specific response, while revealing nothing about responses that are specific for additional epitopes in the same virus. |
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http://hiv-web.lanl.gov/content/hiv-db/REVIEWS/articles/Safrit.html
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| | Tetramers an emerging tool for immunology research |
| | Tetramer technology applies basic principles of immunology to measure the body's response to a foreign invader. | | | For a vaccine to work, these killer T-cells must be present at sufficient levels, and a tetramer assay tells immunologists if this has been achieved. | | | In contrast, because the tetramer assay is so quick and robust, it can be applied to samples from large numbers of patients, and some of the data acquisition can be automated." |
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http://www.emory.edu/EMORY_REPORT/erarchive/2000/January/erjanuary.31/1_31_00tetramers.html
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| | Member Spotlight - Gerry Nepom, Director of the ITN Tetramer Core Facility |
| | The ITN Tetramer Core has the potential to measure the efficacy of investigational therapy within weeks rather than months or even years. | | | While many new diagnostic tools are expensive and initially unavailable to the average patient, tetramers cost about $12 per patient, so developing tetramer technology for clinical applications could turn out to be a healthcare bargain. | | | Tetramer names like 0401GAD and 0415GAD hardly convey their importance in understanding the immune response. |
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http://www.immunetolerance.org/eXpress/research/2003-2/nepom.html
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| | Emory Vaccine Center :: Tetramer |
| | Under contract by the National Institute of Allergy and Infectious Diseases, the Tetramer Core develops tetramer reagents that are not available from commercial sources. | | | The cores are funded through various sources, and hence function somewhat differently, but all are vital to the vaccine research process at Emory and at other institutions throughout the U.S. and the world. | | | The Emory Vaccine Center has five scientific cores, each specializing in a particular research function such as laboratory assays or vaccine trials management. |
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http://www.vaccines.emory.edu/cores/tetramer.shtml
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| | Frequently Asked Questions |
| | If you experience problems with your tetramer, please refer to specific question in the section. | | | We send these tetramers out with a very high expectation of success. | | | However, the tetramers that we have had the most experience with are stable at 4C for greater than 6 months. |
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http://www.yerkes.emory.edu/TETRAMER/faq.html
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| | Immunomics: Benefits |
| | Can be used in conjunction with enzyme-linked immunospot (Elispot), intracellular cytokine analysis (ICC), and other assays | | | Data are interpreted using standard flow cytometry techniques (c) | | | The information submitted will be subject to our |
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http://www.immunomics.com/benefits.asp
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| | Oligomers in plastics packaging. Part 1: Migration tests for vinyl chloride tetramer Food Additives and ... |
| | Drink spiked with tetramer at 30 µg/kg showed a recovery of 70-120% and therefore it may be concluded that migration had not occurred above the 10 µg/kg limit of detection. | | | From a knowledge of the concentration of oligomers, other than the tetramer, in typical PVCs, and from basic migration theory (Pugh 1994), it is possible to estimate the potential for these other oligomers to migrate using the tetramer as a guide. | | | Three tetramer isomers can be observed chromatographically and two have been isolated and characterized (Dawkins et al. |
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http://www.mindfully.org/Plastic/Oligomers-Plastics-Packaging.htm
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| | Tetramer Technologies, L.L.C :: Bringing Creativity to Light |
| | Our business model is to focus only on developing polymeric or oligomeric materials through synthesis, structure/activity development, thorough characterization and close research development relationships with device or other downstream customers. | | | Tetramer Technologies was formed in 2001 as a faculty-driven start-up company commercializing high value research activities pursued at Clemson University. | | | Tetramer Technologies, L.L.C :: Bringing Creativity to Light |
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http://www.tetramertechnologies.com
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| | Tetramer Facility Guide - General Guidelines |
| | Their use for direct clinical applications or commercial purposes is prohibited. | | | Tetramers and/or their derivatives are provided for research purposes only. | | | Feedback forms are necessary for evaluating the quality of tetramer reagents produced at the NIH Tetramer Core Facility, as well as any technical problems or discoveries encountered by users. |
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http://www.niaid.nih.gov/reposit/tetramer/genguide.html
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| | Timothy M. Lohman |
| | Gabriel Waksman and Dr. Srinivasan Raghunathan, we have determined a 2.9 angstrom resolution crystal structure of the tetrameric DNA-binding domain of the E. | | | Each dimer within the tetramer is formed between two monomers, each contributing three | | | Only two of the subunits of the tetramer interact with ss-DNA in the (SSB) |
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http://biochem.wustl.edu/~lohman
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| | Protein Chemistry Core Laboratory - MHC Tetramer Laboratory |
| | The suggested titer for the MHC tetramer reagent is 1:100. | | | Protein Chemistry Core Laboratory - MHC Tetramer Laboratory | | | With some tetramers but not most), their binding to the antigen-specific T lymphocytes is influenced by CD8 participation. |
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http://research.bcm.tmc.edu/protein/mhcstaining.html
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| | Invitrogen - Molecular Probes - Note 7.3 - Technical Focus: MHC Tetramer Technology |
| | CTL-response assays using tetramer technology often detect a response rate that is 50–500 times higher than detected by the LDA method, | | | Similar methods using class II MHC tetramers are also being employed to explore the response of helper (CD-4 expressing) T cells. | | | MHC tetramers are formed by first refolding MHCs in the presence of high concentrations of the desired antigenic peptide, followed by biotinylation of the carboxy-terminus of one chain of the MHC molecule. |
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http://probes.invitrogen.com/handbook/boxes/1642.html
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| | Sloan-Kettering - Tetramer |
| | A typical working concentrations is 1:40, but it is critical to determine the optimal concentration for each tetramer. | | | It is our goal to produce tetramers within 2 to 3 weeks after a request is placed. | | | However, the tetramer facility director or manager may, on occasion, have to change the order due to special circumstances. |
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http://www.mskcc.org/mskcc/html/14259.cfm
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| | Spartanburg.com news: Tetramer Technologies |
| | COMSET is the only university research and development lab in the region focused on developing new optical materials. | | | Tetramer was formed in February 2001 by professors in Clemson's Center for Optical Materials Science and Engineering Technologies. | | | The faculty position is a cornerstone of Clemson University's plan to invest $70 million over the next five years at the Clemson Research Park to make the Upstate a magnet for the advanced materials industry cluster. |
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http://www.spartanburg.com/news/tetramer.html
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| | Flu HA Tetramer Staining |
| | Tetramer may also need to be centrifuged to remove any particulate matter prior to taking an aliquot for staining. | | | b) All new lots of tetramer should be titrated to determine the appropriate concentration for staining. | | | Use NAIVE cells for titration as the IYSTVASSL tetramer does not stain effectors. |
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http://www.northnet.org/sadams/Docs/ClassIprotocolAPC.html
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| | Tetramer Facility Guide - Overview |
| | In order to help the NIH Tetramer Facility prioritize these efforts, please complete the class II epitope survey and tell us which alleles are most valuable for your research. | | | Tetramer Facility staff are currently subcloning a number of class II genes for future tetramer production, including six mouse I-A alleles, and a number of human alleles. | | | In the coming months, additional mouse, human, and non-human primate MHC class II alleles should be available, and (3) Tetramers with an expanded range of fluorophores. |
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http://www.niaid.nih.gov/reposit/tetramer/overview.html
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| | Search Results for tetramer - Encyclopædia Britannica |
| | Many proteins with molecular weights of more than 50,000 occur in aqueous solutions as complexes: dimers, tetramers, and higher polymers—i.e., as chains of two, four, or more repeating basic... | | | About 50 percent of the proteins of egg white are composed of ovalbumin, which is easily obtained in crystals. | | | Normal adult hemoglobin (Hb A) consists of globin containing two pairs of chains of amino acids, of which the alpha chain consists of... |
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http://www.britannica.com/search?query=tetramer&submit=Find&source=MWTEXT
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| | 1-Decene, Tetramer, Mixed with 1-Decene Trimer, Hydrogenated; Robust Summaries & Test Plans; EPA/OPPT/High ... |
| | The print quality is much better than that of the web pages. | | | EPA is posting submissions to EPA related to the HPV Challenge Program for the purposes of making them more easily accessible to the public and inviting public comment on them. | | | 1-Decene, Tetramer, Mixed with 1-Decene Trimer, Hydrogenated; Robust Summaries and Test Plans; EPA/OPPT/High Production Volume Challenge Program (HPV) |
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http://www.epa.gov/chemrtk/1dectmix/c13433tc.htm
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