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| | Thomas F. Holzman |
 | | These drugs are made by teams of researchers. | | | As I indicated, I work at Abbott Laboratories, a large, multinational, health care firm. | | | My group does both basic and applied research. |
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http://www.mcb.arizona.edu/careers/holzman.html
(1960 words)
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| | 521-322 Protein Biochemistry and Proteomics |
| | Laboratory skills and practical management of the experimental program; written research report(s); written assignment of up to 2000 words or a 2-hour written examination (to be advised at the commencement of the subject). | | | In addition to these specific skills, students will develop an appreciation of the current scientific literature and acquire problem-solving abilities in a collaborative setting. | | | Topics covered include modern biochemistry methods and database analysis for characterising protein structure and function; experiments that explore the thermodynamics of protein unfolding; determination and analysis of small molecular weight ligand binding to proteins; and the use of computers in the analysis and homology modeling of protein structure. |
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http://www.unimelb.edu.au/HB/2004/subjects/521-322.html
(263 words)
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| | LABORATORY OF BIOCHEMISTRY |
| | Laboratory of Biochemistry, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892-4255. | | | The Laboratory of Biochemistry has a long tradition of commitment to basic research. | | | Tenured investigators in the Laboratory of Biochemistry are: |
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http://rex.nci.nih.gov/RESEARCH/basic/biochem/biochempage.htm
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| | Gala Biotech |
| | Knowledge of protein purification and analysis techniques and methodologies. | | | Experience of protein purification and analysis techniques and methodologies preferred. | | | Gala Biotech, a business unit of Cardinal Health, a biotechnology company engaged in the production of recombinant proteins in cell culture systems for pharmaceutical and biotechnology applications, seeks a conscientious individual to fill a Protein Biochemistry Technician position at our Middleton facility. |
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http://www.gala.com/jobdescriptions/protbiochem_tech.html
(273 words)
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| | Biochemistry at UConn |
| | The research of the faculty can be categorized into 2 broad areas: protein/peptide structure and macromolecular interactions. | | | Interferon host defense pathway; HIV integrase; HIV gp41/gp120 envelope. | | | Judith A. Kelly -- Protein structure and function using techniques of X-ray diffraction, kinetic methods and interactive computer graphics. |
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http://www.mcb.uconn.edu/research/biochem/biochem.html
(1420 words)
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| | Biochemistry PhD Program |
| | Students participate in research early in their graduate work, preferably in the first year of study, and engage actively in a broadly based seminar and colloquium program. | | | The interdisciplinary science of biochemistry draws from fundamental chemical theory and research techniques as the basis for explorations into problems of biology. |
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http://web.gc.cuny.edu/biochemistry
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| | Cellular and Molecular Biology Course Descriptions - University of New Haven |
| | This course examines the major anabolic and catabolic pathways which are remarkably well conserved in all living organisms. | | | This will provide a theoretical understanding of methods commonly utilized for protein/peptide analysis. | | | These proteins are of critical importance to the development of drugs to control the function of cells. |
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http://www.newhaven.edu/departments/CMB/Course.html
(871 words)
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| | The Virtual Biochemistry Laboratory |
| | Biochemistry is the study of life processes at a molecular level. | | | This Virtual Biochemistry Laboratory consists of several rooms, each focusing on one particular method used in biochemistry: "Nuclear Magnetic Resonance", "Electrophoresis", "Chromatography", "Protein Sequencing", "X-ray Diffraction" and "Protein Folding". | | | The different methods help scientists find out more about molecules, for example proteins. |
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http://www.nobel.se/chemistry/educational/vbl/index.html
(111 words)
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| | Bibliography of |
| | Electron transfer in nitrogenase analyzed by marcus theory: evidence for gating by MgATP. | | | Biochemical and genetic analysis of the nifUSVWZM cluster from Azotobacter vinelandii. | | | Electron allocation to alternative substrates of Azotobacter nitrogenase is controlled by the electron flux through dinitrogenase. |
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http://www.ksu.edu/bchem/gbg/ldlab/bibiol.html
(3940 words)
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| | TOas, Ph.D. |
| | Protein folding dynamics: Quantitative comparison between theory and experiment. | | | subtilis P protein is unfolded under physiological conditions but can be refolded by the addition of small molecule ligands at micromolar concentrations. | | | Protein folding; structure-function relationships of proteins; multidimensional NMR structure determination of macromolecules. |
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http://www.biochem.duke.edu/oas/oas.html
(621 words)
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| | Protein Biochemistry Section |
| | Prepared by Zoraida S. Villadiego, Laboratory of Biochemistry, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892. | | | Laboratory of Biochemistry, Center for Cancer Research, National Cancer Institute, National Institutes of Health | | | Yawen Bai's research focus on understanding proteins' function and to design new proteins with new functions, also to learn the protein folding mechanism on how proteins folds, as well as to understand the basic principles that control their uniqueness and stability of these structures. |
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http://rex.nci.nih.gov/RESEARCH/basic/biochem/pbsect.htm
(148 words)
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| | Pre-Clinical Biochemistry Contract Laboratory offers Protein Production and Purification, Protein Analysis, Enzyme ... |
| | The addition of stabilizing reagents is methodically studied so that bioactivity and physical conformation is not affected. | | | Deglycosylation can be performed as well as further analysis. | | | Proteins can be separated for further purification by isoelectric focusing (IEF) in slab gels in which the movement of proteins through pores in a polyacrylamide gel matrix is controlled by a pH gradient created by soluble ampholytes. |
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http://www.marinbio.com/biochem2.html
(1572 words)
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| | Dagmar Ringe |
| | Different methods are being used to study these systems, including traditional kinetic and structural methods, and low-temperature and time-resolved x-ray structural methods. | | | Our interests are generally in the relationship of protein three-dimensional structure to chemical function. | | | The objectives are to learn how to re-engineer these catalysts to perform useful chemical reactions which may not occur efficiently with the naturally occurring enzyme, to dissect the individual steps in a mechanism and characterize them structurally, or to learn how to inhibit an enzyme specifically and selectively. |
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http://www.bio.brandeis.edu/faculty01/ringe.html
(1525 words)
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| | Peptides and Proteins |
| | The outcome is an elevation in serum cholesterol levels and increased propensity toward the development of atherosclerosis. | | | This technique utilizes a negatively charged detergent (sodium dodecyl sulfate) to denature and solubilize proteins. | | | This chromatographic technique is based upon the use of a porous gel in the form of insoluble beads placed into a column. |
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http://web.indstate.edu/thcme/mwking/protein-structure.html
(3027 words)
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| | The Molecular Structure of Green Fluorescent Protein |
| | Absorption spectra of the hybrid pigments responsible for anomalous color vision. | | | Positions 154 and 164 are on the surface of the protein and may exert their effects through improved solubility and/or reduced aggregation. | | | The structure of GFP has been solved using seleniomethionyl-substituted protein and multi-wavelength anomalous dispersion (MAD) phasing methods. |
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http://www-bioc.rice.edu/Bioch/Phillips/Papers/gfpbio.html
(4825 words)
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| | Unusual Property of Prion Protein Unfolding in Neutral Salt Solution |
| | The results in the literature suggest that the glycine group has unfavorable interaction with solvent water, and its favorable or unfavorable interaction with a solute most probably determines whether a particular protein will be denatured or stabilized by the solute (49, 50) | | | All proteins studied in the literature behave similarly to lysozyme, which is also observed with the prion protein fragment (38). | | | However, a detailed study of the behavior of prion protein in various salt solutions in different environmental conditions, viz., pH, would be useful for understanding the process of unfolding of the molecule. |
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http://pubs.acs.org/cgi-bin/jtextd?bichaw/41/36/html/bi025886t.html
(5950 words)
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| | Walsh/Neurath Lab References 1980 - |
| | A multidisciplinary approach for understanding steroid-protein interaction and its physiological role. | | | Primary structure of duck amyloid protein A. The form deposited in tissues may be identical to its serum precursor. | | | Gut mucosal mast cells in Nippostrongylus-primed rats are the major source of secreted rat mast cell protease II following systemic anaphylaxis. |
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http://faculty.washington.edu/walsh/References.html
(5691 words)
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| | Proteomics and protein biochemistry / Protein expression, extraction and purification |
| | Protein extraction from whole tissues for isoelectric focusing | | | The method of choice depends completely on the stability of the protein and on when and how you want to use it later on. | | | In general it is important to avoid storage conditions that are close to the stability limits of the protein (e.g. |
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http://www.biowww.net/browse-52.html
(817 words)
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| | Protein Biochemistry: Evidence for Charged Amino Acids in Proteins |
| | Over what pH range do only positive charges exist on casein? | | | A protein's shape and structure are in part determined by its balance of positive, negative, and neutral charges. | | | The number of positive charges on casein appears to be equal to the number of negative charges when the solubility of casein is between 4.2 and 5-3. |
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http://www.accessexcellence.org/AE/AEC/AEF/1995/masterman_biochemistry.html
(901 words)
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| | Science/AAAS Collections: : Biochemistry |
| | Find out more about why this message is appearing and what you can do to make your experience on this site better. | | | Home > Collections > Science Subject Collections > Biochemistry | | | Science's SAGE KE New in the Science of Aging |
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http://www.sciencemag.org/cgi/collection/biochem
(128 words)
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| | Protein Synthesis |
| | All three of these processes require specific proteins, some of which are ribosome associated and some of which are separate from the ribosome, but may be temporarily associated with it. | | | Control of translation by heme is clinically important only in erythrocytes. | | | There are several signal transduction pathways whose activations lead to phosphorylation of 4E-BPs. |
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http://web.indstate.edu/thcme/mwking/protein-synthesis.html
(4311 words)
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| | University of Colorado at Boulder :: Chemistry and Biochemistry :: D Wuttke |
| | These studies further our understanding of the biology of the systems studied as well as provide insights into the fundamental nature of protein structure and function. | | | This dimerization may be involved in the organization of the replication complex. | | | We are using the fragment complementation of proteins to analyze the interactions that stabilize protein structures. |
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http://www.colorado.edu/chem/DEC/people/wuttked.html
(1452 words)
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| | 521-322 Protein Biochemistry and Proteomics |
| | The content will include modern biochemistry methods and database analysis for characterising protein structure, function and expression; experiments that emphasise aspects of protein unfolding, determination and analysis of ligand binding data; and the use of computers in the analysis and prediction of protein structure. | | | Special requirements: Before the commencement of the semester, students must advise the Department of Biochemistry and Molecular Biology of their order of preference for the alternative practical sessions and the other subjects they will be taking. | | | Biochemistry and molecular biology 521-211, 521-212 and either 521-220 or 521-221; or 521-024; or 521-213 plus physiology 536-250. |
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http://www.unimelb.edu.au/HB/2001/subjects/521-322.html
(253 words)
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| | CHEM 260 – PROTEIN BIOCHEMISTRY |
| | The source of this research is primary research articles from a variety of journals such as Biochemistry and Journal of Biological Chemistry. | | | Lecture on enzymes (with literature examples of experiments on kinetics and catalysis), lecture on serine proteases, lecture on HIV and HIV protease. | | | Chem 260 is a journal-based course, meaning we will focus on the primary research literature to understand protein structure and function. |
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http://www.chem.csus.edu/lmr/CHEM260syllabus.html
(1591 words)
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| | David P. Cistola, M.D., Ph.D |
| | Arachidonate potentiates NMDA receptor activity and may contribute to the excitotoxicity and neurodegeneration associated with severe neurological disorders such as Alzheimer's and Huntington's diseases. | | | Our laboratory is investigating the structural biology of several families of lipid- and drug-binding proteins. | | | N assignments and chemical shift-derived secondary structure of intestinal fatty acid-binding protein. |
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http://biochem.wustl.edu/~cistola
(1071 words)
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| | UCL Biochemistry Biocomputing Group |
| | Atlas of Protein Side-Chain Interactions - analysis of sidechain-sidechain interaction geometries. | | | PROMOTIF - program to analyse protein structural motifs. | | | Protein-DNA Interactions - structural families of DNA-binding protein, classified by recognition motif. |
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http://www.biochem.ucl.ac.uk/bsm/biocomp
(355 words)
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| | Donald M |
| | Circular dichroism (CD) spectroscopy is a sensitive technique for studying the structures of proteins and nucleic acids. | | | A G-quadruplex structure was directly bound by g5p dimers to form the initiation complex. | | | A decreased binding affinity of Y34F compared with that of wild type protein suggests that Tyr-34, which is located near the interface of cooperatively bound dimers, may be involved in stabilizing the dimer-dimer interface and that its hydroxyl may make an important contribution to the cooperativity factor, w [4]. |
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http://nsm1.utdallas.edu/bio/dgray/gray98.htm
(1427 words)
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| | Directory Institute of Biosciences and Engineering Rice University |
| | Genetic regulation is an essential function in all organisms and provides the ability to respond to signals the reflect environmental conditions, determine developmental processes, and communicate other information within an organism. | | | Each of these genetic regulatory proteins provides specific, crucial functions for the cell, and our experiments are designed to dissect the multiple interactions in which these key proteins participate at the molecular level. | | | "Allosteric Transition in Lactose Repressor Protein." Department of Health and Human Services: National Institutes of Health. |
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http://dacnet.rice.edu/Depts/IBB/directory/index.cfm?FDSID=13
(784 words)
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| | AllRefer.com - protein (Biochemistry) - Encyclopedia |
| | Hundreds of protein molecules have been isolated in pure, homogeneous form; many have been crystallized. | | | protein, any of the group of highly complex organic compounds found in all living cells and comprising the most abundant class of all biological molecules. | | | Proteins are large molecules with high molecular weights (from about 10,000 for small ones [of 50–100 amino acids] to more than 1,000,000 for certain forms); they are composed of varying amounts of the same 20 amino acids, which in the intact protein are united through covalent chemical linkages called peptide bonds. |
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http://reference.allrefer.com/encyclopedia/P/protein.html
(246 words)
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| | Re: Can we predict the function of a protein by its amino acid sequence ? |
| | On the other hand the biological function is closely related to the protein structure and conformational changes accompanying the protein activity. | | | These programs are powerful tools in such fields like protein modelling, protein engineering, theoretical protein biochemistry, bioinformatics etc. The other group are matrices and algorithms generated for comparative analysis of protein homology, multiple alignment, study of protein similarity, variability, evolution, fragments essential for their activity etc. | | | But the question is if we are able to predict theoretically protein function by knowing its primary structure only. |
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http://www.madsci.org/posts/archives/1999-03/922807611.Bc.r.html
(369 words)
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| | Lecomte Lab |
| | Lecomte, J.T.J., Unger, S.W., and La Mar, G.N. (1991) Practical Considerations for the Measurement of the Homonuclear Overhauser Effect on Strongly Relaxed Protons in Paramagnetic Proteins. | | | Strain PCC 7002: a Photosystem I Protein that Shows Structural Homology with SH3 Domains. | | | Unger, S.W., Lecomte, J.T.J., and La Mar, G.N. (1985) The Utility of the Nuclear Overhauser Effect for Peak Assignment and Structure Elucidation in Paramagnetic Proteins. |
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http://research.chem.psu.edu/jtlgroup/publications.html
(1477 words)
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| | Mark E. Dumont - Faculty Page |
| | Genomic approaches to membrane protein structure and function: Although membrane proteins constitute 20-30% of the coding potential of genomes, methods for analyzing their structures and functions are still primitive compared to the approaches available for studies of soluble proteins. | | | G protein signaling: G protein coupled receptors mediate cellular responses to a variety of sensory stimuli, hormones, growth factors, and neurotransmitters and are targets of a number of widely prescribed drugs. | | | We are currently analyzing classes of mutations that can provide information on the structure of the α-factor receptor, on interactions between receptors, and on the mechanism of activation of G proteins by receptors. |
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http://dbb.urmc.rochester.edu/bcbp/members/faculty/Dumont_Mark.html
(720 words)
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| | BIOCHEMISTRY II - PROTEIN SYNTHESIS |
| | Sometimes an extra arm with a variable number of ribonucleotides. | | | Phosphorylation of eIF2 proteins regulates the initiation process. | | | Ribosomes depleted of nearly all protein still form peptide bonds, but removal of 23S rRNA is essential for peptidyl transferase activity. |
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http://www.sbuniv.edu/~ggray/CHE4104/b2c34lecB.html
(1556 words)
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| | Dr. Carl Frieden's Reference Page |
| | Kim, Keehyuk, Ragulan Ramanathan, and Carl Frieden (1997) Intestinal Fatty Acid Binding Protein: A specific residue in one turn stabilizes the native structure and is responsible for the slow refolding. | | | Li, H. and Frieden, C. Phenylalanine side chain behavior of the intestinal fatty acid binding protein: The effect of urea on backbone and side chain stability. | | | Keehyuk Kim, David P. Cistola, and Carl Frieden (1996) Intestinal Fatty Acid-Binding Protein: The Structure Aand Stability of a Helix-less Variant. |
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http://www.biochem.wustl.edu/cflab/ref.html
(1099 words)
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| | The Protein Society: www.proteinsociety.org |
| | Recipients of four other 2006 Society awards will also be acknowledged at Plenary Award Sessions. | | | An opening plenary lecture by Dr. Roger Tsien (Howard Hughes Medical Institute – University of California, San Diego) as well as a closing lecture by Dr. Peter Schultz (The Scripps Research Institute) will be featured along with two Awards Plenary Sessions. | | | The 20th Annual Symposium will introduce a new program for our sponsors and attendees: “The Protein Scientist Recruiting Forum.” Attendees will have the opportunity to submit resumes and research summaries in PDF format to representatives from sponsoring entities who are seeking employees. |
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http://www.proteinsociety.org/pages/page00g.htm
(186 words)
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| | Laboratory Web Page |
| | Investigation of the [4Fe-4S]1+ Cluster Environment of Nitrogenase | | | Protein Without MgATP Hydrolysis: Characterization of a Tight | | | Iron Protein: Defining the Function of Aspartate 129. |
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http://bmbiris.bmb.uga.edu/wlanzilo/Publications.htm
(715 words)
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| | Biochemistry/Amino Acids & Protein Protocols |
| | Selenium can be used for phase determination in multi-wavelength anomalous diffraction (MAD) method. | | | Derivitization of amino groups to remove the positive charge characteristics of the protein, for example to determine the importance of amino groups to a protein's function. | | | For protein concentration, gas pressure is applied directly to ultrafiltration cell. |
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http://www.protocol-online.org/prot/Biochemistry/Amino_Acids___Protein
(515 words)
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| | A Hybrid Algorithm for Determining Protein Structure |
| | I also discuss how future work in AI and other sciences might meet the challenge of the protein folding problem. | | | [28] R.H. Lathrop, T.A. Webster, and T.F. Smith, "Ariadne: Pattern-Directed Inference and Hierarchical Abstraction in Protein Structure Recognition," Comm. | | | [4] J.-F. Gibrat, J. Garnier, and B. Robson, "Further Developments of Protein Secondary Structure Prediction Using Information Theory," J. |
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http://csdl2.computer.org/persagen/DLAbsToc.jsp?resourcePath=/dl/mags/ex/&toc=comp/mags/ex/1994/04/x4toc.xml&DOI=10.1109/64.336147
(687 words)
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| | C. Robert Matthews, PhD - BMP Faculty - UMass Medical School |
| | Gloss L.M., Matthews C.R. Urea and thermal equilibrium denaturation studies on the dimerization domain of Escherichia coli Trp repressor. | | | Gloss, L.M., Matthews C.R. Mechanism of folding of the dimeric core domain of Escherichia coli trp repressor: a nearly diffusion-limited reaction leads to the formation of an on-pathway dimeric intermediate. | | | Steinbach, P.J., Ionescu, R., Matthews, C.R. Analysis of Kinetics Using a Hybrid Maximum-Entropy/Nonlinear-Least-Squares Method: Application to Protein Folding. |
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http://www.umassmed.edu/bmp/faculty/matthews.cfm?start=Publications
(797 words)
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| | Macromolecules Links |
| | Molecular Modeling: A Method of Unraveling Protein Structure and Function | | | The Medical Biochemistry Page: Chemistry of Nucleic Acids | | | The Biology Project: Energy, Enzymes, and Catalysis Problem Set |
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http://bama.ua.edu/~hsmithso/class/bsc_495/macromolecules/macromolecule.html
(53 words)
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| | Invitrogen - Molecular Probes - Proteomics and Protein Biochemistry Products - Stain and Quantitate Phosphoproteins in ... |
| | Simplify your phosphoprotein detection experiments even further by using our Pro-Q Diamond phosphoprotein gel destaining solution. | | | A Discovery Platform for the Analysis of Protein Expression | | | Taking the ratio of the signals from each stain allows estimation of the number of phosphates on the protein, and makes it possible to distinguish between an abundant protein with one or a few phosphate groups and a rare protein with many phosphate groups. |
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http://probes.invitrogen.com/lit/catalog/1/sections/7262.html
(372 words)
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| | Elsevier.com - Current Advances in Protein Biochemistry |
| | It enables pure and applied scientists to keep abreast of the ever-increasing literature being published in their subject area, by providing a subject categorized listing of titles, authors, bibliographic details and authors' addresses. | | | Current Advances in Protein Biochemistry is a monthly current literature searching service which is fast, comprehensive, economical and easy to use. |
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http://www.elsevier.com/locate/caprobio
(147 words)
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| | Protein Characterization Tools |
| | SAPS - Sequence-based Protein Characterization (Statistical Analysis) Service - ISREC (Switzerland) | | | Heart-2DPAGE - Human Myocardial 2-D Electrophoresis Protein Database - German Heart Institute (Germany) | | | ASC - Analytic Surface Calculations (Surface/Volume) of PDB Protein Structures - EMBL-Heidelberg (Germany) |
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http://restools.sdsc.edu/biotools/biotools11.html
(349 words)
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| | Proteomics and protein biochemistry |
| | Tools include: [Protein identification and characterization] [DNA -> Protein] [Similarity searches] [Pattern and profile searches] [Post-translational modification prediction] [Topology prediction] [Primary structure analysis] [Secondary structure... | | | Protocols The following protocols are available in PDF format: Isotope Coded Affinity Tag Reagents Acid Cleavable ICAT Labelling Prot... |
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http://www.biowww.net/browse.php?cat=5
(204 words)
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| | Listmania! Protein Biochemistry books I need to buy |
| | MRI and NMR: extensive reading list: A list by Junqian Xu, MR Physical Chemist | | | The other protein NMR bible, written by the good folks at Genentech | | | The protein NMR bible - written by the man himself |
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http://www.amazon.com/exec/obidos/tg/listmania/list-browse/-/2HLZDR8UBQNJ6
(200 words)
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