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| | PCBE: White Paper on Alternative Sources of Pluripotent Stem Cells -- Full Report |
 | | Crucial to this approach is (a) enunciating a concept of organismic death of an early embryo and (b) devising criteria that permit a determination that embryonic death has occurred. | | | And if, in the process, definite criteria could be established for distinguishing between the not-human and the human, this proposal might have the salutary effect of erecting boundaries that would open avenues for scientific advance without threatening human dignity, boundaries that do not now govern the practices of human embryo research in the private sector. | | | Others, however, doubt the wisdom of exposing the prospective child (while an embryo) to a hazardous procedure merely for the sake of some hypothetical future benefit, or of encouraging parents to practice embryo biopsy simply or mainly as a source of “personalized” stem cells should their future child someday have need of them. |
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http://bioethicsprint.bioethics.gov/reports/white_paper/text.html
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| | Biotech New World |
| | ASC and related therapeutic approaches are currently undergoing clinical trials or being used in the treatment of cancers, stroke, autoimmune diseases, anemia, bone and cartilage deformities, corneal scarring and skin grafts, to name a few. | | | Last, it should be pointed out that ASC regenerative medicine, in the form of bone marrow transplants, has in fact been used to treat leukemia and certain cancers for many years. | | | But before ESCs call be used in humans, two major problems must be overcome: tumor formation and autoimmune rejection, problems that do not appear to exist with ASC therapies. |
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http://www.catholiceducation.org/articles/medical_ethics/me0071.html
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| | Every Thought Captive: Stem Cell Update: Bone Marrow |
| | But pluripotency is a possibility as a few researchers have pointed out. | | | Parents can elect to keep an umbilical chord for their child (and the mother apparently), bone marrow is used, placents are possible replacements for SCs, cells from women's arms are being use to cure incontinence...etc. The point being that the very use of ASCs does not require pluripotency. | | | Pluripotency is the term to describe the ability of a stem cells to develop into any kind of cell - e.g. |
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http://steigerblog.blogspot.com/2005/01/stem-cell-update-bone-marrow.html
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| | Crucial gene found for embryonic stem cell maintenance |
| | Further analysis by Dr. Labosky and colleagues revealed that the previously identified critical regulators of embryonic pluripotency (Oct4 and Fgf4) appear to be properly expressed in Foxd3-mutant embryos. | | | This finding suggests that Foxd3 functions either downstream of Oct4 and Fgf4, or in a parallel pathway to regulate pluripotency in the early stages of mammalian embryogenesis. | | | This work represents a significant advance in our understanding of the complex genetic framework that supports early mammalian development and stem cell pluripotency. |
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http://www.eurekalert.org/pub_releases/2002-10/cshl-cgf101002.php
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| | PCBE: White Paper Conference Call With Reporters May 12 |
| | This end is, in the view of the Council, a desirable goal for our society and one that justifies, we think, making the extra effort to seek out, to assess, and to attempt new, ethically uncontroversial methods of stem cell derivation. | | | Further, as members of a national bioethics body we are mindful of the need to understand and respect the strongly held ethical views of our fellow citizens even when we do not share them. | | | Now the report conducts what can only be, at this stage, a preliminary hearing of these proposals, an analysis of their respective strengths and weaknesses, ethical, scientific, and practical, with special attention to the ethical analysis. |
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http://www.bioethics.gov/reports/white_paper/press_conference.html
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| | Chris C Mooney |
| | I refer you to two recent articles that I've done that touch on this subject, here and here. | | | National Review's Kelly misleading cites a range of the flawed studies mentioned above, as adult stem cell advocates always do. | | | First of all, Kelly's notion that an emphasis on embryonic stem cell research will divert resources away from adult stem cell research isn't supported by the facts. |
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http://www.chriscmooney.com/blog.asp?Id=1281
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| | CAGT - Thomas Zwaka, MD, PhD |
| | In this way, our laboratory hopes to make important new advances towards a greater understanding of pluripotency and its use in medical and nonmedical settings. | | | The underlying concept of pluripotency provides not only a unique basis for our understanding of the molecular processes involved in the cellular specification of the early embryo in vivo, but also for our understanding of the basic biology of pluripotent cell lines derived from mammalian pre-implantation embryos in vitro. | | | The long-term goal of our laboratory is to develop a greater understanding of ES cell pluripotency in mouse and human ES cells, which offer unlimited materials for investigating the molecular mechanisms of pluripotency. |
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http://www.bcm.edu/genetherapy/faculty/zwaka.htm
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| | Cloning Fact of June 20 2002 by Americans to Ban Cloning |
| | Their research is published today in the peer-reviewed journal Nature (Y. Jiang et al. | | | Verfaillie and her team successfully put the adult bone marrow cells to what is considered the "gold standard" test for pluripotency (i.e. | | | The MAPC's could be directed in culture to form specific tissues from each of the three "primary germ layers", which are the three main developmental types of tissues -- an indication that the cells can potentially form any tissue |
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http://www.cloninginformation.org/info/cloningfact/fact-02-06-20.htm
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| | Hans R. Scholer, Ph.D. |
| | Area 5: Structure - function relationship for Oct4 dimers [see b]. | | | Area 4: Artificial ways to reprogram somatic cell nuclei and to generate autologous ESC and molecular approaches to re-establish pluripotency. | | | Our scientific hypothesis is that ESCs, ASCs, and germ cells can be interconverted and that expression of Oct4 is essential for some of the underlying processes. |
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http://www.med.upenn.edu/crrwh/Scholer.html
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| | Stem Cell Type Comparison: Advantages and Disadvantages |
| | Adult blood stem cells have been very useful for treating patients who need blood regeneration, but adult blood stem cells have not been used (until recently) to treat patients who need heart, brain, lung or liver regeneration. | | | However, researchers are investigating such mechanisms, as the ability to control or regulate these processes could be key to the repopulation and repair of diseased tissue. | | | With growing proof of their pluripotency, and without the danger of forming teratomas, adult stem cells actually offer a greater possibility for successful clinical therapies than do embryonic stem cells. |
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http://www.cellmedicine.com/comparison.asp
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| | Embryonic Stem Cell - Pluripotency Today's Stem Cell Research |
| | A new drug that activates the Wnt pathway maintains the undifferentiated state of pluripotent human and mouse embryonic stem cells. | | | add a third pathway, showing that activation of the canonical Wnt signaling pathway may be sufficient to keep cells undifferentiated and expressing markers of pluripotency (such as Oct-4 and Nanog). | | | The extent to which there is cross-talk or convergence between the pathways is not yet known. |
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http://www.stemnews.com/archives/000177.html
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| | Pluripotency and tumorigenicity - Nature Genetics |
| | -catenin in pluripotent cells supports the notion that many cancers may result from the dysregulation of stem-cell programs. | | | But when the molecular pathways that control stem cells go awry, the result can often be the development of tumors. | | | Pluripotent stem cells are essential for embryonic development and regeneration of adult tissues. |
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http://www.nature.com/cgi-bin/doifinder.pl?URL=/doifinder/10.1038/ng1202-557
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| | Department of Obstetrics, Gynecology & Reproductive Sciences at University of Pittsburgh |
| | First, the fundamental nature of the ability of hESCs to differentiate into any tissue in the body, pluripotency, requires the capacity to express new genetic programs during development. | | | Sammak’s laboratory is focused on two aspects of hESC biology. | | | We are investigating the structural and functional underpinnings of pluripotency in nuclear function and plasticity. |
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http://obgyn.medicine.pitt.edu/personnelDetail.asp?pid=587&id=67&ptype=2&pnavcat=2
(388 words)
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| | Presentation |
| | This has paved the way to research aimed at using ES-derived cells for cell replacement therapies. | | | Our research objectives are (i) to unravel the mechanisms underlying self-renewal and pluripotency of mouse and non-human primate ES cells, and (ii) to design new experimental strategies aimed at coaxing ES cell differentiation into specialized cells of clinical interest. | | | There is a growing body of evidence that regulatory mechanisms in the ES mitotic cycle differ markedly from those regulating the somatic mitotic cycle. |
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http://www.ens-lyon.fr/LBMC/savatier/pres-uk.htm
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| | Embryonic Stem Cell |
| | An embryonic stem cell is pluripotent in nature, giving it the ability to develop into any of the 200 types of cells required by the human body. | | | Earlier studies into pluripotency were focused on mice, where it was discovered that extracted mouse stem cells continued to divide even when in culture dishes, and still managed to differentiate into any cell type when tested under the right conditions. | | | Some challenges researchers now face are to cultivate isolated embryonic stem cells past the first stages of development, to decrease probabilities of stem cell implants being rejected by the host body, and to gain control over the multiplication of the stem cells so as not to produce cancer. |
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http://www.iscid.org/encyclopedia/Embryonic_Stem_Cell
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| | Pluripotency - Wikipedia, the free encyclopedia |
| | In the members of Kingdom Animalia, pluripotent stem cells can develop into any of the three major tissue types: endoderm (interior gut lining), mesoderm (muscle, bone, blood), and ectoderm (epidermal tissues and nervous system). | | | Pluripotent stem cells can eventually specialize in any bodily tissue, but they cannot themselves develop into a human being. | | | In cell biology, a pluripotent cell is one able to differentiate into many cell types. |
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http://en.wikipedia.org/wiki/Pluripotency
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| | ZOOGI + Master regulators to the pluripotency of stem cells + |
| | The therapeutic goal then is to take these blank slates and coax them into, say, liver or brain tissue. | | | Now, researchers at Whitehead Institute for Biomedical Research working with human embryonic stem cells have uncovered the process responsible for the single-most tantalizing characteristic of these cells: their ability to become just about any type of cell in the body, a trait known as pluripotency. | | | But in order to guide them out of pluripotency with efficiency, we need to know what keeps them there to begin with. |
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http://www.zoolgi.com/map/showthread.php?t=2104
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| | Molecule that restricts mouse cells' potency could yield embryonic stem cells without the sacrifice of embryos |
| | Embryonic stem cells’ scientific appeal lies in their pluripotency: they have not yet determined their ultimate role, so each has the potential to become one of more than 200 tissue types in the body. | | | The work is the first demonstration of a mechanism by which pluripotency is lost in mammalian embryos, one that operates with nearly the precision of an on/off switch in mouse embryos. | | | Schöler, also the director of Penn’s Center for Animal Transgenesis and Germ Cell Research, said GCNF is the first factor known to repress the key gene Oct4, which is expressed in pluripotent embryonic cells. |
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http://www.eurekalert.org/pub_releases/2001-12/uop-mtr120301.php
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| | Research - The Institute of Human Genetics - University of Newcastle |
| | The pluripotency, and compliancy to genetic manipulation, of embryonic stem cells confers great potential for their use in various clinical applications, particularly for the repair of damaged tissues. | | | These and other differences suggest that although human and mouse ES cells share some common markers of pluripotency, the function and downstream signalling of these markers may differ. | | | The aim of this project is to identify new pluripotency factors in human ES cells. |
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http://www.ncl.ac.uk/ihg/research/project/841
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| | CRISP - Computer Retrieval of Information on Scientific Projects, Abstract Display |
| | Therefore, the establishment of molecular standards and global molecular knowledge of their pluripotency is an obligatory step toward the design of rational clinical treatments. | | | 2- Characterization of the role of signaling pathways underlying pluripotency, by (i) testing the activity of a novel small compound GSK3a inhibitor, BIO, in vivo in mouse embryos; (ii) addressing the role of TGFa signaling in this process; and (iii) performing an unbiased expression cloning to identify molecular factors involved in self-renewal in HESCs. | | | Abstract: DESCRIPTION (provided by applicant): Embryonic stem cells are pluripotent cells derived from the epiblast of the early embryo. |
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http://crisp.cit.nih.gov/crisp/CRISP_LIB.getdoc?textkey=6817371&p_grant_num=1R01GM073379-01&p_query=&ticket=11594733&p_audit_session_id=53272482&p_keywords=
(358 words)
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| | Feeder-free System For Maintaining Pluripotency In Embryonic Stem Cells Pioneered |
| | This new system for maintaining pluripotency could be a providential break for basic researchers and clinicians investigating the potential of HESCs, as it is a potential first step in providing an unlimited source of tissue transplant if HESCs' potential comes to fruition in clinical medicine. | | | Feeder-free System For Maintaining Pluripotency In Embryonic Stem Cells Pioneered (January 12, 2004) -- Rockefeller University researchers in collaboration with two European scientists, have devised a system for maintaining existing or new human stem cell lines that excludes the need for troublesome... | | | Now, Rockefeller University researchers in collaboration with two European scientists, have discovered a way around this problem: they've devised a system for maintaining existing or new human stem cell lines that excludes the need for troublesome mouse feeder cells. |
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http://www.sciencedaily.com/releases/2004/01/040108075614.htm
(990 words)
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| | [No title] |
| | Both teams put forward models proposing a cooperative effect between Oct4 and Nanog, suggesting that each functions in maintaining pluripotency by suppressing differentiation of specific pathways. | | | A novel protein in a novel pathway maintains pluripotency in embryonic stem cells | | | Nanog conferred the ability for self-renewal on ES cells in a manner that was independent of, but augmented by, the presence of LIF, which was independent of Oct3/4 expression and which was resistant, but not refractory, to the effects of differentiation-promoting agents. |
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http://www.mult-sclerosis.org/news/Jun2003/NovelProteinandPathwayKeepsStemCellsYoung.html
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| | Undergraduate Research Program |
| | Currently, together with undergraduate students, we take the correlation generated from the microarray and test whether change in expression of genes identified in the microarray are responsible for pluripotency. | | | There is general interest to learn how cells become pluripotent and to identify the gene products responsible for pluripotency. | | | Our recent finding indicates that imaginal disc cells of Drosophila are a good model system to study this problem. |
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http://www.washington.edu/research/urp/opp/biology.html
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| | Forever Young: Digging for the roots of stem cells: Science News Online, Sept. 17, 2005 |
| | Besides discovering that pivotal role for these regulators, the researchers mapped out the molecular biology behind pluripotency. | | | This work, Boyer predicts, will provide more insights both about pluripotency in stem cells and about the remarkable process by which a single fertilized cell becomes an entire organism. | | | And with knowledge of the mechanics behind pluripotency, she says, scientists might learn to reprogram a mature cell so that it, too, could have the pluripotency of a stem cell. |
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http://www.sciencenews.org/articles/20050917/fob6.asp
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| | Gene Regulatory Networks in Early Mouse Embryo and Stem Cells |
| | The goal of this research is to understand the fundamental mechanism for the maintenance of self-renewal, immortality, and pluripotency, which will help in the adaptation of stem cells to replacement therapy for aging and dysfunctional cells and organs. | | | Studies will utilize the potential of "embryogenomics" approaches, including DNA microarrays, embryo manipulations, and bioinformatics to analyze global gene expression regulations in mouse preimplantation embryos and stem cells. | | | Preimplantation embryo; Stem cells; Embryo manipulation; DNA Microarrays; EST; Gene regulatory network; Embryogenomics; Bioinformatics; Pluripotency |
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http://www4.nationalacademies.org/pga/rap.nsf/ByTitle/54.10.07.B5818?OpenDocument
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| | PGC Pluripotency and Proliferation |
| | They are: 1) stem cell factor, a growth factor; 2) the bFGF gene, expressed along the PGC migration route; and 3) cytokines of the interleukin/LIF family; each with their own respective receptor. | | | Leukemia inhibitory factor (LIF) promotes the undifferentiated, pluripotent phenotype of ES cells. |
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http://www.brown.edu/Courses/BI0032/embgerm/pgc4.html
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| | McLaren Group |
| | Addition of FGFs later does not support the transition, suggesting that FGF receptors may be down-regulated. | | | Our research focuses on mouse primordial germ cells (PGCs) and the epigenetic changes undergone both by the PGCs themselves, and by the pluripotent stem cells derived from them. | | | The novel gene mRif1, derived from our PGC genetic screen, has a different expression pattern from other pluripotency genes that are expressed in the germ cell lineage. |
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http://www.gurdon.cam.ac.uk/groups/mclaren.html
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| | ScienceWeek |
| | Human blastocyst-derived, pluripotent cell lines are described that have normal karyotypes, express high levels of telomerase activity, and express cell surface markers that characterize primate embryonic stem cells but do not characterize other early lineages. | | | After undifferentiated proliferation in vitro for 4 to 5 months, these cells still maintained the developmental potential to form trophoblast and derivatives of all three embryonic germ layers, including gut epithelium (endoderm); cartilage, bone, smooth muscle, and striated muscle (mesoderm); and neural epithelium, embryonic ganglia, and stratified squamous epithelium (ectoderm). | | | Jiang et al (University of Minnesota, US) discuss stem cell pluripotency, the authors making the following points: 1) Embryonic stem (ES) cells are pluripotent cells derived from the inner cell mass of the blastocyst that can be propagated indefinitely in an undifferentiated state. |
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http://scienceweek.com/2002/sw020927.htm
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| | Keystone Symposia Scientific Conferences on Biomedical and Life Science Topics |
| | Stem cells have generated considerable excitement recently in the scientific, clinical, and public domains. | | | Pluripotent cells derived from early embryos or fetal tissues appear capable of indefinite expansion and have the capacity to differentiate into many cell types. | | | Develop new collaborations among the scientists in attendance and new insights that will lead to novel and effective interventions |
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http://www.keystonesymposia.org/Meetings/ViewMeetings.cfm?MeetingID=655
(1086 words)
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| | Review |
| | Pan GJ, Pei DQ Identification of two distinct transactivation domains in the pluripotency sustaining factor nanog Cell Research 13 (6): 499-502 DEC 2003 | | | The purification of Wnt proteins would allow their use as exogenous purified cytokines in attempts to amplify stem cells before bone marrow transplantation. | | | The homeodomain protein Nanog has been shown to be crucial for the embryonic stem cell renewal and pluripotency. |
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http://www.jcmm.ro/content.jsp?pageId=368
(377 words)
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| | Leukemia Inhibitory Factor [LIF] , recombinant human : LIF1010 |
| | LIF has a number of other activities including cholinergic neuron differentiation, control of stem cell pluripotency, bone and fat metabolism, mitogenesis of certain factor dependent cell lines and promotion of megakaryocyte production in vivo. | | | Leukemia Inhibitory Factor (LIF) is a lymphoid factor which promotes long-term maintenance of embryonic stem cells by suppressing spontaneous differentiation. |
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http://www.apoptag.com/Product/ProductDataSheet.asp?ProductItem=LIF1010
(322 words)
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| | Merriam-Webster Online |
| | For More Information on "pluripotent" go to Britannica.com | | | Get the Top 10 Search Results for "pluripotent" | | | : not fixed as to developmental potentialities : having developmental plasticity stem cell> |
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http://www.m-w.com/cgi-bin/dictionary?va=pluripotent
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| | ZFG 2002 |
| | Vodička, P. Motlík, J. Proteasome activity is required for proper chromosome segregation in meiosis I. In: Čejkovice 2002 From Oocyte to Embryonic Stem Cell: A Lesson from Pluripotency. | | | - S. [From Oocyte to Embryonic Stem Cell: A Lesson from Pluripotency. | | | - S. [From Oocyte to Embryonic Stem Cell: A lesson from Pluripotency. |
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http://www.lib.cas.cz/www/asep/data/ZFG02.HTM
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