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Topic: Ploidy



  
 ploidy
DNA ploidy by image analysis of individual foci of prostate cancer: a preliminary report.
Since CaP is often a heterogeneous and multifocal disease, it doesn't necessarily follow that a tiny piece of tissue obtained on needle biopsy is representative of the lesion as a whole.
DNA ploidy measurements in PCa: differences between image analysis and flow cytometry and clinical implications.
http://www.prostatepointers.org/prostate/oppenheimer/oppenheimer1.html   (841 words)

  
 Prostate Cancer DNA Ploidy and Response to Salvage Hormone Therapy After Radiotherapy With or Without Short-Term Total ...
Gauwitz MD, Pollack A, El-Naggar AK, et al: The prognostic significance of DNA ploidy in clinically localized prostate cancer treated with radiation therapy.
Visakorpi T, Kallioniemi O-P, Paronen IYI, et al: Flow cytometric analysis of DNA ploidy and S-phase fraction from prostatic carcinomas: Implications for prognosis and response to endocrine therapy.
DNA ploidy was related to overall survival in multivariate analysis,
http://www.jco.org/cgi/content/full/21/7/1238   (4770 words)

  
 Ploidy
: DNA Ploidy and surgically treated prostate cancer.
It can be performed on needle aspiration, core biopsies, TURP chips or radical prostatectomy specimens using either flow cytometry measurements (FCM) or static image cytometry (SIC).
DNA ploidy analysis: This analysis is available commercially in the U.S.A. as a routine test.
http://www.prostatepointers.org/prostate/ploidy.html   (581 words)

  
 The A-Type Cyclin CYCA2;3 Is a Key Regulator of Ploidy Levels in Arabidopsis Endoreduplication -- Imai et al. 18 (2): ...
but was specific to the change of ploidy levels.
Ploidy Distribution Analysis of Transgenic Plants Ectopically Expressing CYCA2;3-GFP or mDB-CYCA2;3-GFP.
also affects the ploidy levels finally achieved in these organs.
http://www.plantcell.org/cgi/content/full/18/2/382   (7997 words)

  
 Flow cytometric ploidy analysis of testicular biopsies from sperm-negative wet preparations -- Coskun et al. 17 (4): ...
Lee, S.E. and Choo, M.S. (1991) Flow-cytometric analysis of testes in infertile men: a comparison of ploidy to routine histopathologic study.
The discrepancies of histopathology with flow cytometric ploidy
correlates wet preparation with flow cytometric ploidy analysis.
http://humrep.oxfordjournals.org/cgi/content/full/17/4/977   (4065 words)

  
 Changes to hepatocyte ploidy and binuclearity profiles during human chronic viral hepatitis -- Toyoda et al. 54 (2): ...
Flow cytometric analysis on echo-guided needle liver biopsy.
characterised hepatocyte ploidy at various stages of chronic
cirrhosis) to clarify the hepatocyte ploidy pattern during chronic
http://gut.bmjjournals.com/cgi/content/full/54/2/297   (3377 words)

  
 VALIDITY OF FLOW CYTOMETRY FOR TESTING PLOIDY LEVEL IN RYEGRASSES
Overall, flow cytometry was shown to be the superior technique for ploidy determination.
In recent years a new technique, flow cytometry, has emerged for ploidy determination.
Flow cytometry was first used to examine cell cycles, DNA content, and ploidy levels in humans and other animals, but by 1982 the technique was also adapted for DNA content determination in plant material.
http://cropandsoil.oregonstate.edu/seed-ext/Pub/1998/SR9919.html   (2503 words)

  
 Different ploidy levels of megakaryocytes generated from peripheral or cord blood CD34+ cells are correlated with ...
MK ploidy was measured by a double-staining technique and flow cytometry (left panels).
MK ploidy was measured by a double-staining technique and flow cytometry, according to a modified procedure of the Cycle Test
in megakaryocytic ploidy is associated with increase in megakaryocytic
http://www.bloodjournal.org/cgi/content/full/99/3/888   (6140 words)

  
 More on ploidy, n, etc (Unit 14)
Haploid gametes (one egg, one sperm) fuse to form diploid zygote.
(Don't confuse it with 'alternation of generations' in Cnidarians, where it is not associated with change in ploidy.)
Note the above definitions involve issues of mitosis, meiosis, and levels of ploidy.
http://www.mun.ca/biology/Help_centre/1001_2_tutorialpages/MoreOnPloidyNetc.html   (681 words)

  
 Ploidy Determination in Agrostis Using Flow Cytometry and Morphological Traits -- Bonos et al. 42 (1): 192 -- Crop ...
Ploidy Determination in Agrostis Using Flow Cytometry and Morphological Traits -- Bonos et al.
This technique will aid breeders to quickly and
Ploidy Determination in Agrostis Using Flow Cytometry and Morphological Traits
http://crop.scijournals.org/cgi/content/abstract/42/1/192   (344 words)

  
 DNA Ploidy and Markovian Analysis of Neoplastic Progression in Experimental Pancreatic Cancer -- Postier et al. 51 (3): ...
DNA Ploidy and Markovian Analysis of Neoplastic Progression in Experimental Pancreatic Cancer -- Postier et al.
Correspondence to: Russell G. Postier, PO Box 26901, Dept. of Surgery, U. of Oklahoma Health Sciences Center, Oklahoma City, OK 73190.
DNA Ploidy and Markovian Analysis of Neoplastic Progression in Experimental Pancreatic Cancer
http://www.jhc.org/cgi/content/abstract/51/3/303   (248 words)

  
 Regulation of megakaryocyte colony forming cell numbers and ploidy by dideoxynucleosides in immunodeficient mice.
The fluorescence of individual cells was then measured using a Perceptics image analysis system.
These findings suggest that AZT may affect physiological processes that lead to platelet formation.
Similarly, AZT treatment changed the modal ploidy number of colony megakaryocytes derived from bone marrows of immunodeficient mice from 16N to 32N.
http://www.aegis.com/aidsline/1994/feb/M9420170.html   (539 words)

  
 Host-parasite interactions and the evolution of ploidy -- Nuismer and Otto 101 (30): 11036 -- Proceedings of the ...
the evolution of ploidy level have focused on the influence
This allowed us to derive very general conditions for the evolution
We took two approaches to analyzing the model.
http://www.pnas.org/cgi/content/full/101/30/11036   (2201 words)

  
 Patient Services - DNA Ploidy Analysis in Prostate Cancer
Ploidy analysis by DIA offers superior accuracy compared with flow cytometry for small amounts of cancer such as may be found in needle biopsies.
Role of DNA Ploidy Analysis in Prostate Cancer: Studies have shown that patients with diploid cancers (having normal DNA content in cancer cells) have longer cancer-free intervals and survival than those with non-diploid content.
The currently used methods of DNA ploidy analysis are still hampered by lack of appropriate controls.
http://www.bostwicklaboratories.com/patientservices/dna.html   (249 words)

  
 Ploidy doubling - Hybridizing Forum - GardenWeb
Fertility for a particular cross, particularly an inter-specific cross, can certainly be improved by changing ploidy.
Get help with it if you want to do it.
She also said that sometimes mixed ploidy crosses are often best done with the lower ploidy as the recipient (female), or perhaps it was the other way round.
http://forums.gardenweb.com/forums/load/hybrid/msg071120348568.html   (1533 words)

  
 Ploidy and Meiosis
Those of you who are really thinking hard about this may have realized that cross-over could confound this picture; however, what we are discussing here is an "ideal" example and what happens in the case of the majority of gene loci of any homologous pair.
The figure below summarizes the process of meiosis and the changes in ploidy that occur along the way.
Only two pairs of chromosomes are shown in this example; however, the ploidies given for each stage (e.g.
http://www.auburn.edu/academic/classes/zy/vert_embryo/html/PloidyandMeiosis.html   (984 words)

  
 DNA Content and Ploidy Determination of Bromegrass Germplasm Accessions by Flow Cytometry -- Tuna et al. 41 (5): 1629 ...
DNA Content and Ploidy Determination of Bromegrass Germplasm Accessions by Flow Cytometry
The objective of this study was to characterize the ploidy of
DNA Content and Ploidy Determination of Bromegrass Germplasm Accessions by Flow Cytometry -- Tuna et al.
http://crop.scijournals.org/cgi/content/abstract/41/5/1629   (392 words)

  
 vgn-ext-hidden_Abstract
TNM stage II (30%) and III (70%) colonic adenocarcinomas had been analyzed for ploidy by flow cytometry and were categorized as diploid or non diploid (aneuploid and tetraploid).
The relationship between ploidy and chromosome 18q allelic loss was also studied.
DNA ploidy is a stronger prognostic variable compared to microsatellite instability or 18q allelic loss in patients with stages II and III colon cancer
http://www.asco.org/ac/1,1003,_12-002636-00_18-0034-00_19-0033762,00.asp   (744 words)

  
 Ploidy101
A nuclear grade can be assessed by any pathologist with enough experience with prostate cancer.
The value that DNA ploidy analysis adds is that is that it is an objective measurement that can be compared between labs using standardized techniques and that can be used to perform a quick check on the "ball-park" accuracy of Gleason scoring.
Studies have shown that tumors that hae a significant aneuploid peak do not behave as well as those that do not.
http://www.prostatelab.com/ploidy101.htm   (424 words)

  
 The effect of cytokines on the ploidy of megakaryocytes -- Teramura and Mizoguchi 8 (4): 245 -- Stem Cells
Department of Medicine, Tokyo Women's Medical College, Japan.
The effects of recombinant cytokines on the ploidy of human megakaryocytes
The effect of cytokines on the ploidy of megakaryocytes
http://stemcells.alphamedpress.org/cgi/content/abstract/8/4/245   (205 words)

  
 Relationship between Chromosomal Instability and Intratumoral Regional DNA Ploidy Heterogeneity in Primary Gastric ...
The purpose of this study was to elucidate the relationship
Although the mortality rate of patients with gastric cancer
Articles by Furuya, T. Articles by Sasaki, K. Clinical Cancer Research Vol.
http://clincancerres.aacrjournals.org/cgi/content/full/6/7/2815   (2927 words)

  
 Ploidy Levels among Species in the 'Oxalistuberosa Alliance' as Inferred by Flow Cytometry -- EMSHWILLER 89 (6): 741 -- ...
of this technique to infer ploidy levels among members of the
However, the use of flow cytometry to infer ploidy level
Flow cytometry has frequently been used in ploidy analysis (e.g.
http://aob.oxfordjournals.org/cgi/content/full/89/6/741   (5757 words)

  
 Genome Duplication Events and Functional Reduction of Ploidy Levels in Sturgeon (Acipenser, Huso and Scaphirhynchus) -- ...
per individual at a given locus provides evidence of ploidy
Ploidy level as inferred from microsatellite analysis for the 20 of the 24–25 extant Acipenseriform species examined in this study
ploidy level in which some loci have diverged sufficiently from
http://www.genetics.org/cgi/content/full/158/3/1203   (5203 words)

  
 Relation of tumor-cell ploidy to survival in children with medulloblastoma -- Gajjar et al. 11 (11): 2211 -- Journal of ...
showed that only tumor-cell ploidy and clinical risk group had a
that both ploidy and clinical risk group are important prognostic factors
Relation of tumor-cell ploidy to survival in children with medulloblastoma
http://www.jco.org/cgi/content/abstract/11/11/2211   (396 words)

  
 Pronuclear Location Before the First Cell Division Determines Ploidy of Polyspermic Pig Embryos -- Han et al. 61 (5): ...
In contrast to 2PN embryos (2 of 22), most blastocysts
Ploidy of 2PN and PPN embryos that developed to blastocysts
Fibroblast cells were established from each fetus, and their ploidy was then examined by a cytological method.
http://www.biolreprod.org/cgi/content/full/61/5/1340   (4789 words)

  
 MOB1, an Essential Yeast Gene Required for Completion of Mitosis and Maintenance of Ploidy -- Luca and Winey 9 (1): 29 ...
When present as a single integrated copy, many of the conditional
may suggest that the mechanism for the increase in ploidy displayed
been unable to study the increase in ploidy in isolation using
http://www.molbiolcell.org/cgi/content/full/9/1/29   (7653 words)

  
 p53 Mutations in L3-Loop Zinc-binding Domain, DNA-Ploidy, and S Phase Fraction Are Independent Prognostic Indicators in ...
The median follow-up time in our study group was 71 months (range
Table 4 Relationships of DNA ploidy to clinicopathological and biological variables of CRCs
Table 3 Relationships of p53 mutations in the highly conserved domains or in the functional/structural domains, and clinicopathological and biological variables of the 160 CRC patients
http://cebp.aacrjournals.org/cgi/content/full/11/11/1322   (5475 words)

  
 Chapter 27: Bryophytes
Question: What is the meiosis life cycle pattern of the bryophytes?
Draw and label the structures of the life cycle of a moss and included or indicate the place or occurrence of the following: First and last cells of both the sporophyte and gametophyte phases; meiosis; mitosis; the ploidy of each structure; and fertilization.
Gametes form from MITOSIS or MEIOSIS (circle one)
http://web.utk.edu/~kdmcfar/bot110/outlines/chapter27.html   (402 words)

  
 Ploidy - Wikipedia, the free encyclopedia
This was one of the procedures used by Japanese researchers to produce Kaguya the fatherless mouse.
This may lead to problems in cell development.
The ploidy of cells can vary within an organism.
http://en.wikipedia.org/wiki/Ploidy   (785 words)

  
 Ploidy, expression of erbB1, erbB2, P53 and amplification of erbB1, erbB2 and erbB3 in non-small cell lung cancer -- ...
The aim of this study was to assess the prognostic value of deoxyribonucleic acid analysis, expression oferbB1, erbB2 and P53, and amplification levels of erbB1, erbB2 and erbB3 in non-small cell lung cancer (NSCLC).
Ploidy, expression of erbB1, erbB2, P53 and amplification of erbB1, erbB2 and erbB3 in non-small cell lung cancer
Amplification of the erbB family does not seem to be a frequent event in non-small cell lung cancer.
http://erj.ersjournals.com/cgi/content/abstract/16/5/991   (580 words)

  
 Flow Cytometric DNA Ploidy Analysis of Peripheral Blood From Patients With Sézary Syndrome : Detection of ...
Flow Cytometric DNA Ploidy Analysis of Peripheral Blood From Patients With Sézary Syndrome
DNA ploidy study by flow cytometry is important for blood-based diagnosis of SS and detection of minimal residual disease in aneuploid SS after treatment.
Detection of aneuploid neoplastic T cells in peripheral blood samples of patients with CTCL is associated with LCT in skin, lymph node, or other tissues.
http://www.ajcp.com/previews/abstracts/204079.html   (353 words)

  
 Seed Laboratory from the September 2004 Crop and Soil News/Notes
Researchers: The test is useful to researchers who need information on the ploidy level of germplasm collections, outcrossing (contamination through pollen from neighboring fields), and for other ploidy research objectives.
There is no doubt that ploidy by cytometry is a technology that can help many people in assuring the ploidy identity of seeds and plants.
The ploidy by cytometry test was used at the Oregon State University Seed Laboratory first to determine the ploidy level of tetraploid ryegrass varieties as a part of the certification process.
http://cropandsoil.oregonstate.edu/newsnotes/0409/seed-lab.html   (986 words)

  
 Dna Ploidy
E Piaton MD, PP Bringuier PhD, MH Ravigneaux MD, P Perrin MD, M Devonec MD Flow cytometric measurements of deoxyribonucleic acid ploidy and proliferative activity for evaluating malignant potential of human bladder cancers
M Haraguchi MD, S Moriguchi MD, D Korenaga MD, T Oshiro MD, T Okuyama MD, H Baba MD, Y Adachi MD, Y Maehara MD, K Sugimachi MD Ploidy independence of nucleoli number in advanced prostatic
Prognostic significance of DNA ploidy in advanced gastric carcinoma-multivariate analysis
http://www.cancerprev.org/Journal/Issues/17/1/21   (215 words)

  
 Partec Flow Cytometry - Ploidy Analyser PA
The ploidy analysis is extremely time and cost efficient.
The Ploidy Analyser measures the DNA content of many thousands of individual nuclei within seconds or minutes.
Ploidy determinations with the Partec PA Ploidy Analyser are performed by the precise measurement of the total DNA content of individual nuclei, which can be derived from almost any plant tissue.
http://www.partec-cytomics.com/products/ploidy.html   (440 words)

  
 Tumour proliferation, angiogenesis, and ploidy status in human colon cancer -- Cristi et al. 58 (11): 1170 -- Journal ...
Tumour proliferation, angiogenesis, and ploidy status in human colon cancer
Tumour proliferation, angiogenesis, and ploidy status in human colon cancer -- Cristi et al.
Copyright © 2005 by the BMJ Publishing Group Ltd & Association of Clinical Pathologists.
http://jcp.bmjjournals.com/cgi/content/abstract/58/11/1170   (302 words)

  
 Ploidy Analysis - Prostate Cancer Research Institute
The landmark paper from the Mayo Clinic in Rochester, Minnesota substantiates the value of the ploidy analysis, although other prostate cancer centers dispute its significance.
The importance of ploidy as an independent prognostic factor is clearly demonstrated by Lerner, et al.
When the data were analyzed below with respect to PSA, GS and ploidy status, a significantly higher rate of disease relapse was seen in patients with non-diploid tumors.
http://www.prostate-cancer.org/education/staging/ploidana.html   (261 words)

  
 PLOIDY IN ACTINIDIA
Flow cytometry has been used to estimate ploidy levels in more than 350 individuals from 24.
The results indicate that no assumptions should be made as to the ploidy of any Actinidia plant.
There is a clear relationship between the DNA content of 2C nuclei and ploidy level.
http://www.actahort.org/books/444/444_7.htm   (115 words)

  
 S-Phase Fraction and DNA Ploidy in 633 T1T2 Breast Cancers: A Standardized Flow Cytometric Study -- Chassevent et al. 7 ...
Articles by Chassevent, A. Articles by Spyratos, F. Clinical Cancer Research Vol.
Comparisons between DNA ploidy or SPF and patient characteristics
The influence of DNA ploidy and SPF on outcome, adjusted for
http://clincancerres.aacrjournals.org/cgi/content/full/7/4/909   (5089 words)

  
 Embryo development and ploidy
Because Maize and arabidopsis exemplify an important difference in how ploidy affects the outcome of crosses in plants.
These changes are of fundamental importance to understand the formation of endosperm, the single most important food source for humans (rice and wheat).
Effect of parental ploidy on the ploidy of the progeny
http://courses.washington.edu/bot427/week3_00.htm   (877 words)

  
 Ploidy Regulation of Gene Expression -- Galitski et al. 285 (5425): 251 -- Science
Sensing, signalling and integrating physical processes during Saccharomyces cerevisiae invasive and filamentous growth.
strains that varied in ploidy from haploid to tetraploid.
Nuclear DNA endoreduplication during petal development in cabbage: relationship between ploidy levels and cell size.
http://www.sciencemag.org/cgi/content/abstract/285/5425/251   (1451 words)

  
 Cell cycle kinetics and DNA ploidy in head and neck squamous cell cancer (HNSCC) (Meeting abstract).
The state of the art may have changed since the publication date.
These results should be considered in designing kinetically based clinical trials for HNSCC.
DNA ploidy and BUDR-LI were not significantly correlated with tumor site, clinical stage or pathological grading but DNA-aneuploid tumors showed shorter Tpot (4.1 days) than DNA-diploid ones (8 days; p less than 0.05).
http://www.aegis.com/aidsline/1995/dec/M95C3231.html   (446 words)

  
 Botany online: Evolution: The Modern Synthesis - Polyploidy
All degrees of ploidy between diploidy and decaploidy exist.
Galium anisophyllum covers all evenly numbered degrees of ploidy between diploidy and decaploidy.
It seems instead, as if the increase in ploidy provides the plant with more flexibility in expressing its genome.
http://www.biologie.uni-hamburg.de/b-online/e37/37d.htm   (882 words)

  
 Variation in DNA-ploidy Levels of Reynoutria Taxa in the Czech Republic -- MANDÁK et al. 92 (2): 265 -- Annals ...
The most common ploidy levels of particular Reynoutria taxa
taxa and ploidy levels are often far from each other (Fig. 1).
mainly by the ploidy level of these taxa.
http://aob.oxfordjournals.org/cgi/content/full/92/2/265   (2804 words)

  
 Identifying Ploidy - OrchidTalk Forums
Including one on ploidy, for those who aren't sure what this question was, not to mention the answer.
Some people also say that polyploid plants generally have thicker and possibly wider leaves, although other people say wider leaves means better form.
http://www.rv-orchidworks.com/orchidtalk/showthread.php?t=185   (889 words)

  
 Karyotype analysis of pm by Y and ploidy by LSC for...
M Ohmori MD PhD, H Miki MD PhD, S Kobayashi MD PhD, E Ohmori MD, M Nakano CT Dept Pathology, Kagawa Medical University, Kagawa 761-07, Japan
Karyotype analysis of pm by Y and ploidy by LSC for...
METHODS: We analyzed the nuclear ploidy by laser scanning cytometer (LSC) and detected the 149-bp Y-chromosome-specific gene, Kogan segment (Y) by the PCR method using human Y-chromosome-specific primer from deparaffinized specimens of 16 PM diagnosed by histological criteria.
http://www.cancerprev.org/Journal/Issues/20/5/304/1145   (312 words)

  
 Chromosomal Variation
Ploidy refers to the number of chromosome sets found in a cell.
We have encountered variations of this word before: haploid, which describes cells with one set of chromosomes, and diploid, which describes cells with two sets of chromosomes.
(The opposite term, euploidy - "good ploidy", refers to chromosome sets with the correct number of chromosomes.) Aneuploidy usually results from nondisjunction during meiosis.
http://www.emunix.emich.edu/~rwinning/genetics/variat.htm   (666 words)

  
 ImagePath Systems Products Listing
This system also is optimized for full color image capture at resolutions of 1315 x 1024 pixels - which provides detail approaching the lens resolution of the microscope.
The ImagePath 300 consists of a vertically integrated system for performing rapid image-based DNA Ploidy Analysis with simple, intuitive, menu-driven instructions...
ImagePath Systems™ provides vertically integrated imaging products and reagents for the anatomic pathology laboratory.
http://www.imagepath.com/products.html   (184 words)

  
 Ploidy
One would think that that would be called "ploid", but instead, it is "haploid" (half of the normal ploidy), or 1N.
Polyploid is just a fancy word for any multiple of the normal (diploid) ploidy.
In the normal case, two gametes combine to form a zygote, which has, once again, a diploid (2N) set of chromosomes.
http://www.bedfordorchids.com/ploidy.htm   (707 words)

  
 Fungi HO
Study this overview, and note the ploidy of each life cycle stage.
Below is a diagram similar to the one on the previous page, but with three major life cycle stages replaced by "A", "B" and "C".
What is the name of A? What is its ploidy?
http://fig.cox.miami.edu/~blase/fungi.htm   (829 words)

  
 DNA Ploidy Analysis, Body Fluids
This test should not be used as a diagnostic test for cancer.
Blood should not be submitted for DNA ploidy analysis.
The DNA index is a measure of the nuclear DNA content of tumor cells compared to normal cells.
http://www.labcorp.com/datasets/labcorp/html/chapter/mono/tg004600.htm   (214 words)

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