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Topic: Metaphase



  
 Metaphase fragility of the human RNU1 and RNU2 loci is induced by actinomycin D through a p53-dependent pathway -- Yu ...
Metaphase fragility of the human RNU1 and RNU2 loci is induced by actinomycin D through a p53-dependent pathway -- Yu et al.
http://hmg.oxfordjournals.org/cgi/reprint/7/4/609   (22 words)

  
 Metaphase to Anaphase (mat) Transition-defective Mutants in Caenorhabditis elegans -- Golden et al. 151 (7): 1469 -- ...
During metaphase of meiosis I, the six bivalents are often distinguishable as a "pentagonal array" or, in side view, as a cluster of three axially aligned bivalents (C).
The metaphase to anaphase transition is a critical stage of
With a focus on the metaphase to anaphase transition,
http://www.jcb.org/cgi/content/full/151/7/1469   (8351 words)

  
 Prognostic Value of Metaphase-Fluorescence In Situ Hybridization in Follow-up of Patients With Acute Myeloid Leukemia ...
Furthermore, metaphase-FISH is a quantitative technique that analyzes a large number of cells, up to 2,000 metaphases per sample, a feature which helps to evaluate the clinical significance of the finding and is useful in planning further treatment.
Each slide contained on average 400 metaphases suitable for the analysis after in situ hybridization.
Only clear unbroken diploid metaphase-plates with no overlapping metaphases/nuclei were analyzed.
http://www.bloodjournal.org/cgi/content/full/89/9/3330   (4029 words)

  
 Lack of Checkpoint Control at the Metaphase/Anaphase Transition: A Mechanism of Meiotic Nondisjunction in Mammalian ...
metaphase I this behavior appeared to be related to the failure
Thus, we conclude that the most likely explanation for our observations is that a chromosome-mediated checkpoint mechanism
Aneuploidy study of human oocytes first polar body comparative genomic hybridization and metaphase II fluorescence in situ hybridization analysis
http://www.jcb.org/cgi/content/full/139/7/1611   (6726 words)

  
 Mitosis
The interval between nuclear envelope breakdown and anaphase onset includes prometaphase and metaphase, and requires approximately 33 minutes.
For more information about these experiments, see S.
At several points during metaphase, the chromatid arms may unwind from each other.
http://www.life.umd.edu/CBMG/faculty/wolniak/wolniakmitosis.html   (1098 words)

  
 Metaphase Typewriter
Ordinary awareness is one of the biggest mysteries of our age: scientists are totally baffled by the fact that humans enjoy "inner experience" along with their behavior and are at a loss to explain the origin of this experience tho much progress has been made in explaining the behavior.
Members of the Consciousness Theory Group felt that there was something vaguely unethical about possessing an already occupied body and wondered if we could create an empty "consciousness-friendly" vessel and invite wandering souls to occupy it.
Further information about metaphase devies and their future extensions may be found in Nick Herbert's "Elemental Mind: Human Consciousness and the New Physics".
http://members.cruzio.com/%7Equanta/meta.html   (512 words)

  
 MetaPhase - Band page with free MP3 music downloads on SoundClick
I feel as an artist I'd like to push the sound forward (while respecting the past) as much as possable; that's basically what MetaPhase means.
Phase, a specific process in which an event(s) takes place.
In my case, Metaphase is my process of production and performance- always trying new ways to edit, produce, direct, and perform.
http://www.soundclick.com/metaphase   (551 words)

  
 Proper metaphase spindle length is determined by centromere proteins Mis12 and Mis6 required for faithful chromosome ...
A similar set of experiments was done with mis6-302.
Composite motifs and repeat symmetry in S. pombe centromeres: Direct analysis by integration of NotI restriction sites.
similar to metaphase and anaphase A in higher eukaryotes is crucial
http://www.genesdev.org/cgi/content/full/13/13/1664   (7388 words)

  
 Involvement of the cohesin Rad21 and SCP3 in monopolar attachment of sister kinetochores during mouse meiosis I -- ...
the inner domain of metaphase I centromeres beneath the associated
SCP3 and SCP2 are located beneath closely associated sister kinetochores at the inner centromere domain colocalizing with INCENP and aurora-B kinase
From middle diplotene up to metaphase I there is a continuous loss of SCP3/Rad21 from chromosome arms, and an accumulation at centromeres, that in turn change their structure.
http://jcs.biologists.org/cgi/content/full/117/7/1221   (7427 words)

  
 Cell cycle behavior of human HP1 subtypes: distinct molecular domains of HP1 are required for their centromeric ...
Cell cycle behavior of human HP1 subtypes: distinct molecular domains of HP1 are required for their centromeric localization during interphase and metaphase -- Hayakawa et al.
change their intracellular localization from interphase to metaphase.
Cell cycle behavior of human HP1 subtypes: distinct molecular domains of HP1 are required for their centromeric localization during interphase and metaphase
http://jcs.biologists.org/cgi/content/full/116/16/3327   (5281 words)

  
 Under arrest: cytostatic factor (CSF)-mediated metaphase arrest in vertebrate eggs -- Tunquist and Maller 17 (6): 683 ...
of the pathway for the CSF-mediated establishment of meiotic metaphase
E/Cdk2 pathways is sufficient to cause metaphase arrest.
treatment." (2) Causing "the nuclei [to] arrest...in metaphase."
http://www.genesdev.org/cgi/content/full/17/6/683   (8718 words)

  
 Olympus Microscopy Resource Center: Fluorescence Microscopy Image Gallery - Human Metaphase Chromosome Centromeres
Incident light fluorescence microscopy is growing rapidly in importance as an investigational tool in the fields of medical and biological research.
Using in situ hybridization fluorescence microscopy techniques (FISH), the centromere region of human metaphase chromosomes can be distinguished from adjacent areas by selective staining with the appropriate fluorophores.
First, all the chromatin strands make copies of themselves through the process of DNA replication.
http://www.olympusmicro.com/galleries/fluorescence/pages/centromerechromosome1humansmall.html   (494 words)

  
 Degradation of Polyubiquitinated Cyclin B Is Blocked by the MAPK Pathway at the Metaphase I Arrest in Starfish Oocytes ...
Degradation of Polyubiquitinated Cyclin B Is Blocked by the MAPK Pathway at the Metaphase I Arrest in Starfish Oocytes -- Oita et al.
The abbreviations used are: MI, metaphase of the first meiotic
The metaphase state is established by the activity
http://www.jbc.org/cgi/content/full/279/18/18633   (4485 words)

  
 Mitosis Exam
How many tetrads are there in metaphase I of meiosis in human beings?
How many chromatids are seen in a cell in metaphase I of meiosis in a human being?
How many chromosomes (as opposed to chromatids) are there in metaphase II of meiosis in human beings?
http://mama.indstate.edu/pfaffle/genetics/mitosis.html   (471 words)

  
 Mitosis
Cohesin breakdown is caused by a protease called separase (also known as separin).
The interaction of actin and a myosin (not the one found in skeletal muscle) tightens the belt, and the cell is pinched into two daughter cells.
The chromosomes are at their most compact at this time.
http://users.rcn.com/jkimball.ma.ultranet/BiologyPages/M/Mitosis.html   (749 words)

  
 DOC1R: a MAP kinase substrate that control microtubule organization of metaphase II mouse oocytes -- Terret et al. 130 ...
of the MOS/.../MAPK pathway which mediates metaphase II spindle
So we believe that both DOC1R and MISS mediate important
microtubules, we treated metaphase II oocytes with nocodazole,
http://dev.biologists.org/cgi/content/full/130/21/5169   (5495 words)

  
 Requirement of functional telomeres for metaphase chromosome alignments and integrity of meiotic spindles -- Liu et al. ...
vesicle breakdown, and progress through metaphase I (MI), anaphase
Rather, functional telomeres may be involved in mediating metaphase
abnormal behavior of chromosomes and spindles at metaphases
http://emboreports.npgjournals.com/cgi/content/full/3/3/230   (2765 words)

  
 Mad2 and BubR1 Function in a Single Checkpoint Pathway that Responds to a Loss of Tension -- Shannon et al. 13 (10): ...
typical of metaphase kinetochores at 37°C but with slightly slower
in tension is the cause of the prolonged metaphase.
metaphase plate, behavior typical of cells at 37°C. Even though
http://www.molbiolcell.org/cgi/content/full/13/10/3706   (7785 words)

  
 PDM Consultants
Our core strength has been the domain expertise of our consultants coming from Aerospace, Automotive and Consumer Electronics Industry.
JDD-Consult is focused on system design and development with Metaphase 2 as a base.
Metaphase, Info*Engine, JAVA, RUP/Rose, WWW-Interfaces, Legacy System Integrations
http://www.pdmic.com/vendors/consult2.html   (3465 words)

  
 Hypertonicity-Induced Projections Reflect Cell Polarity in Mouse Metaphase II Oocytes: Involvement of Microtubules, ...
Liu JL, Sung LY, Barber M, Yang X. Hypertonic medium treatment for localization of nuclear material in bovine metaphase II oocytes.
A previous study showed that with hypertonic sucrose treatment,
Metaphase II oocytes were incubated in M2 plus 10% (v/v) FBS with different cytoskeletal drugs for 3 h and then in 400 mM sucrose for 30 min.
http://www.biolreprod.org/cgi/content/full/67/6/1853   (6019 words)

  
 Ectopic NORs on human chromosomes 4qter and 8q11: rare chromosomal variants detected in two families -- Guttenbach et ...
A common anomaly revealed by Ag-NOR staining of metaphases from nine cancers.
Microfluorometric detection of deoxyribonucleic acid replication in human metaphase chromosomes.
Prenatal diagnosis identified an unusual chromosome 8 in all metaphases of the fetus.
http://www.jmedgenet.com/cgi/content/full/36/4/339   (1996 words)

  
 Mitosis
Look back through this paragraph and try to.)
During anaphase the pairs of identical chromatids separate at their centromeres and are drawn to opposite ends of the cell by the spindle fibers.
Metaphase ends when the centromeres are ready to separate and allow the chromatids to be drawn to opposite ends of the cell.
http://www.pgjr.alpine.k12.ut.us/science/whitaker/Cell_growth_division/mitosis.html   (494 words)

  
 Effect of Water Stress on Cell Division and Cdc2-Like Cell Cycle Kinase Activity in Wheat Leaves -- Schuppler et al. ...
phases of metaphase, anaphase, and telophase, but omitting prophase
The early response to water stress, a decrease in Cdc2-kinase activity within 3 h, must have derived largely from cells in
prophase and metaphase, since these contain the most active forms
http://www.plantphysiol.org/cgi/content/full/117/2/667   (7845 words)

  
 Metaphase
The analysis of metaphase chromosomes is one of the main tools of cancer cytogenetics.
Additionally, normal Metaphase spreads are used as hybridization matrix for Comparative Genomic Hybridization (CGH) experiments.
Metaphase (from the Greek words μετά = after and φάσις = stage) is a stage of mitosis in the eukaryotic cell cycle in which condensed chromosomes align in the middle of the cell before being separated into each of the two daughter cells.
http://www.freecaviar.com/search.php?title=Metaphase   (209 words)

  
 Genial Metaphase Arresting Solution
About 1000 cultures may be processed using Metaphase Arresting Solution.
Metaphase Arresting Solution is a gentle alternative to commonly used colchicines/colcemid preparations and is less toxic to cells because the concentration of colchicine is reduced while the extra blocking activity of vinblastine sulphate.
The use of Metaphase Arresting Solution is effective in increasing the mitotic indexes of bone marrows, difficult tumor samples, and problematic tissues where colchicine/colcemid alone appers to be ineffective.
http://www.rainbowscientific.com/genial/gen-metaphasearresting.html   (242 words)

  
 BGN 12: Effects of cycloheximide treatments on prophase and metaphase cells of a (2X) Hordeum vulgare X (2X) H. ...
Metaphase cells observed 2 hours after a 1 h treatment with cycloheximide.
These observations suggest that treatment with cycloheximide disturbs the congression of the bulbosum chromosomes onto the metaphase plate and that this may eventually result in the elimination of these chromosomes (Wheatley, Noda and Kasha, unpublished observations).
In some prophase cells, from roots treated with cycloheximide for 2 to 3 hours, a distinct separation of groups of chromosomes was observed.
http://wheat.pw.usda.gov/ggpages/bgn/12/12p72.html   (624 words)

  
 Secured cutting: controlling separase at the metaphase to anaphase transition -- Uhlmann 2 (6): 487 -- EMBO Reports
metaphase by this protease, under conditions in which the separase
in S phase until the end of metaphase.
DNA damage, cells adapt and overcome the metaphase block.
http://emboreports.npgjournals.com/cgi/content/full/2/6/487   (3675 words)

  
 LAI's Metaphase Spread Finding System
Dicentric analysis is done in Giemsa-stained and centromere-painted metaphase-chromosome spreads.
Statistics are immediately available and easily analyzed at the end of each test.
The automatic "postage stamp" display of the low power image allows the researcher to perform a quick quality assessment test, which increases the throughput of the system.
http://www.loats.com/mf-full.html   (728 words)

  
 Multiple Subunits of the Caenorhabditis elegans Anaphase-Promoting Complex Are Required for Chromosome Segregation ...
, 2000  Metaphase to anaphase (mat) transition-defective mutants in Caenorhabditis elegans.
in metaphase of meiosis I, meiosis II, or mitosis, the structures
Surviving animals were allowed to recover for 24 hr and were then transferred to new plates.
http://www.genetics.org/cgi/content/full/160/2/805   (4003 words)

  
 Metasystems GmbH - Metaphase Finder System Metafer / MSearch
Finding a metaphase on the slide is just the first step in the analysis procedure.
, metaphases may be relocated and subsequently captured and analyzed in
Images generated in this way may be easily opened on any external
http://www.metasystems.de/products/metafer/metafer_msearch.htm   (837 words)

  
 SparkNotes: Mitosis: Metaphase and Anaphase
Movement is mediated by the kinetochore microtubles, which push and pull on the chromosomes to align them into what is called the metaphase plate.
You'll flip over our Pre-Algebra Study Cards—writing out flashcards is now a thing of the past.
Microtubule structure allows them to be dynamic molecules.
http://www.sparknotes.com/biology/cellreproduction/mitosis/section2.rhtml   (445 words)

  
 Metaphase Chromosome Tethering Is Necessary for the DNA Synthesis and Maintenance of oriP Plasmids but Is Insufficient ...
HMG1-DBD is not associated specifically with metaphase chromosomes but is dispersed throughout the field of view.
a stage of mitosis immediately after metaphase (11, 12).
Little specific association with metaphase chromosomes was observed,
http://jvi.asm.org/cgi/content/full/77/21/11767   (8503 words)

  
 Nonrandom Distribution of Metaphase AgNOR Staining Patterns on Human Acrocentric Chromosomes -- Héliot et al. 48 ...
Number of AgNOR structures carried by acrocentric chromosomes for individual P. For 34 metaphases observed, a modal value of 9, a mean of 8.4, and an SD deviation of 0.8 were found.
This study has been carried out on 10 acrocentric
metaphases were analyzed, i.e., 68 occurrences per acrocentric
http://www.jhc.org/cgi/content/full/48/1/13   (3396 words)

  
 CiteULike: The similarity of DNA sequences remaining bound to scaffold upon nuclease treatment of interphase nuclei and ...
The similarity of DNA sequences remaining bound to scaffold upon nuclease treatment of interphase nuclei and metaphase chromosomes.
CiteULike: The similarity of DNA sequences remaining bound to scaffold upon nuclease treatment of interphase nuclei and metaphase chromosomes.
Both the method involving restriction endonuclease treatment/1,2/and a novel procedure based on mild staphylococcal nuclease digestion were used.
http://www.citeulike.org/user/textoris/article/326778   (244 words)

  
 Cloned Mice from Fetal Fibroblast Cells Arrested at Metaphase by a Serial Nuclear Transfer -- Ono et al. 64 (1): 44 -- ...
The oocytes that successfully fused with a donor cell
at metaphase at the time of transfer can be reprogrammed, and
fetal fibroblasts at metaphase and used them in our cloning
http://www.biolreprod.org/cgi/content/full/64/1/44   (3827 words)

  
 [No title]
Metaphase 2.0 is available through a network of systems integrators and value added resellers worldwide.
Metaphase 2.0 is an object management system with comprehensive workflow capabilities ranging from simple informal routing to enterprise-wide distribution and workflow processing.
Additional modules provide product structure, configuration management, and image view and markup features.
http://is.twi.tudelft.nl/~hommes/tl1598.html   (117 words)

  
 Metaphase
It is common to observe sister chromatids unwinding at specific points during metaphase (compare image 3 and 4).
This site (2-4) is the metaphase plate, and is obliquely situated (from upper left to lower right) in this particular cell.
Prometaphase and metaphase in a stamen hair cell of Tradescantia virginiana.
http://www.life.umd.edu/CBMG/faculty/wolniak/MetaTRAD.html   (88 words)

  
 Follicle-Stimulating Hormone Affects Metaphase I Chromosome Alignment and Increases Aneuploidy in Mouse Oocytes Matured ...
Procedures) Act of 1986 and associated codes of practice.
of the metaphase spindle, there is pole-to-pole movement of
The concentration of FSH did, however, have a significant effect
http://www.biolreprod.org/cgi/content/full/72/1/107   (6962 words)

  
 Chromosome Metaphase
metaphase includes process chromosomes align on metaphase includes process equatorial plane...
metaphase followed by anaphase metaphase stage in which find replicated chromosome metaphase includes...
The nucleus usually has no apparent structure, but during cell division (metaphase), the DNA appears
http://www.e-dna-tests.info/resources/chromosome-metaphase.html   (276 words)

  
 meiosis
It is during this alignment that chromatid arms may overlap and temporarily fuse (chiasmata), resulting in crossovers
Here is where the critical difference occurs between Metaphase I in meiosis and metaphase in mitosis.
Although this drawing from the text shows the chromosomes pairs in close association, including the chiasmata or points of contact of the arms of adjacent chromosomes, this is characteristic only of very late Prophase I and is more common in Metaphase I. Metaphase I
http://taggart.glg.msu.edu/bs110/meiosis.htm   (577 words)

  
 CELL DIVISION: Meiosis...
Metaphase I is when tetrads line-up along the equator of the spindle.
Metaphase II is similar to mitosis, with spindles moving chromosomes into equatorial area and attaching to the opposite sides of the centromeres in the kinetochore region.
Spindle fibers attach to the centromere region of each homologous chromosome pair.
http://www.emc.maricopa.edu/faculty/farabee/BIOBK/BioBookmeiosis.html   (1469 words)

  
 Production of identical sextuplet mice by transferring metaphase nuclei from four-cell embryos -- Kwon and Kono 93 ...
Four-cell-stage donor embryos were synchronized at metaphase with nocodazole, fused individually with ooplasts, and artificially
how and when donor metaphase nuclei are reprogrammed and acquire
Unfertilized metaphase II oocytes have the potential to induce reprogramming of nuclei from cleavage-stage embryos, making
http://www.pnas.org/cgi/content/full/93/23/13010   (2438 words)

  
 Microtubule Flux Mediates Poleward Motion of Acentric Chromosome Fragments during Meiosis in Insect Spermatocytes -- ...
Direct Visualization of Microtubule Flux during Metaphase and Anaphase in Crane-Fly Spermatocytes
Maloriented Bivalents Have Metaphase Positions at the Spindle Equator with More Kinetochore Microtubules to One Pole than to the Other
prometa- or metaphase were cut with the laser, the acentric fragments
http://www.molbiolcell.org/cgi/content/abstract/12/12/4054   (512 words)

  
 The Kinesin-like Calmodulin Binding Protein Is Differentially Involved in Cell Division -- Vos et al. 12 (6): 979 -- ...
Each bar represents the observation time (not necessarily the beginning and end of a mitotic phase), except for the bars representing interphase, which were continued to show the observed completion of cell division.
which cells were observed in prometaphase or metaphase after
Clearly visible are the differences in the lengths of prophase and metaphase and the variation in the length of telophase between KCBP-Ab–injected cells and their controls.
http://www.plantcell.org/cgi/content/full/12/6/979   (5892 words)

  
 Meiosis: Meiosis II: Metaphase II
This is in contrast to metaphase I, in which homologous pairs of chromosomes align on the metaphase plate.
Concept 11: Meiosis II: Metaphase II The chromosomes become arranged on the metaphase plate, much as the chromosomes do in mitosis, and are attached to the now fully formed spindle.
• Single chromosomes align on the metaphase plate, much as chromosomes do in mitosis.
http://www.phschool.com/science/biology_place/biocoach/meiosis/metaii.html   (117 words)

  
 Molecular Cytogenetics and Genome Organization - Pat Heslop-Harrison
To determine the chromosome constitution of Bronco 90, C-banding and fluorescent in situ hybridization techniques were applied to somatic and meiotic metaphase chromosomes.
Chromosomal variation in Crocus vernus Hill (Iridaceae) investigated by in situ hybridization of rDNA and a tandemly repeated sequence.
After individual chromosome identification at metaphase I with the pAs1 probe, we found the occurrence of pairing between chromosomes of different homoeology groups.
http://www.le.ac.uk/biology/phh4/titleabst.htm   (13464 words)

  
 Genetic Analysis of Early- versus Late-Stage Ovarian Tumors -- Shridhar et al. 61 (15): 5895 -- Cancer Research
Metaphase spreads from normal human lymphocytes were prepared
Hoque M. T., Ishikawa F. Human chromatid cohesin component hRad21 Is phosphorylated in M phase and associated with metaphase centromeres.
http://cancerres.aacrjournals.org/cgi/content/full/61/15/5895   (6877 words)

  
 PG-IV: P87 - Cell Synchronization and Metaphase Chromosome Isolation from Rice and Other Cereals for Flow Karyotyping ...
Cell cycle progress of root tip meristems were monitored by flow cytometric analysis of nuclei suspension prepared form root tips at one hour intervals during hydroxyurea treatment.
Suspensions of metaphase chromosomes were prepared from synchronized root tips by simple chopping in a chromosome stabilizing buffer, stained with propidium iodide and analyzed on FACScan analyzing flow cytometer.
When majority of the root-tip cells reached late S-phase or early G2 phase, seedlings were exposed to microtubule inhibitors for 4-6 h for accumulation of large number of cells at metaphase.
http://www.intl-pag.org/4/abstracts/p87.html   (262 words)

  
 genome.gov Talking Glossary: "metaphase"
Dr. Paul Meltzer, of the National Human Genome Research Institute's Laboratory of Cancer Genetics, defines metaphase.
The phase of mitosis, or cell division, when the chromosomes align along the center of the cell.
Because metaphase chromosomes are highly condensed, scientists use these chromosomes for gene mapping and identifying chromosomal aberrations.
http://www.genome.gov/glossary.cfm?key=metaphase   (66 words)

  
 BIOdotEDU
This allows the proteins of the kinetochore to form, which in turn attach to the spindle fibers.
Since the chromosome is attached to two sets of fibers, pulling in opposite directions, the whole chromosome structure is tugged into the center of the cell, where all of the kinetochores of all the chromosomes line up.
At the end of metaphase, all the kinetochores are lined up on the equatorial plane, facing in opposite directions, and attached to the opposite poles of the cell by means of spindle fibers.
http://www.brooklyn.cuny.edu/bc/ahp/LAD/C9/C9_metaphase.html   (643 words)

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