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| | Enzyme Linked Immunosorbent Assay |
 | | The Enzyme Linked Immunosorbent Assay (ELISA), (Enzyme Immunoassay (EIA) or Solid-Phase Immuosorbent Assay (SPIA)) is a sensitive laboratory method used to detect the presence of antigens (Ag) or antibodies (Ab) of interest in a wide variety biological samples. | | | The conjugate could be a commercial prepartion specific for the Ag of interest, or an in-house conjugated monoclonal or polyclonal Ab, or even patient serum. | | | The conditions used for the coating of the solid phase with Ag or Ab can seriously effect the outcome of the assay. |
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http://www.uq.edu.au/vdu/ELISA.htm
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| | Patent 4999286: Sulfate reducing bacteria determination and control |
| | An antiserum was prepared against the enzyme tentatively identified as 1,2-propanediol dehydrogenase from Neisseria bacteria and used in an immunoassay to detect the presence of said enzyme in lysates of bacterial samples. | | | Sulfate reducing bacteria are detected by preparing a lysate so as to release an enzyme essential to derive energy by reduction of sulfate such as adenosine 5'-phosphosulfate reductase (APS reductase), containing the lysate with an antibody for the enzyme, and detecting the presence of the reaction product of the enzyme and the antibody. | | | The presence, and if present, preferably the quantity of sulfate-reducing bacteria are determined by a very specific immunoassay, and a nondeleterious but effective amount of a bactericidal or bacteriostatic agent is introduced into the aqueous environment to control sulfate-reducing bacteria which are detected. |
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http://www.freepatentsonline.com/4999286.html
(310 words)
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| | HSV 1 & 2 Information |
| | Enzyme Immunoassay for the qualitative determination of GM Class antibodies to Herpes Simplex Virus type 1 & 2. | | | Enzyme Immunoassay for the quantitative determination of IgG Class antibodies to Herpes Simplex Virus type 2. | | | Enzyme Immunoassay for the quantitative determination of IgG Class antibodies to Herpes Simplex Virus type 1 & 2. |
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http://www.equipar.it/hsv.htm
(310 words)
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| | Auk, The: Corticosterone metabolites can be measured noninvasively in excreta of European Stonechats (Saxicola torquata rubicola) |
| | High-performance liquid chromatography of excreta of a female and a male stonechat injected with tritiated corticosterone showed that corticosterone is excreted in the form of numerous metabolites and that the corticosterone enzyme-immunoassay crossreacted with most of those metabolites. | | | The objective of this study was to validate an enzyme immunoassay (EIA) to quantify corticosteroid metabolites in excreta of European Stonechats (Saxicola torquata rubicola). | | | Therefore a non-invasive technique was developed to measure metabolites of corticosterone in excreta of European Stonechats (Saxicola torquata rubicola) using a corticosterone enzyme-immunoassay. |
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http://www.findarticles.com/p/articles/mi_qa3793/is_200210/ai_n9143192
(310 words)
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| | HSV 1 & 2 Information |
| | Enzyme Immunoassay for the qualitative determination of GM Class antibodies to Herpes Simplex Virus type 1 & 2. | | | Enzyme Immunoassay for the quantitative determination of IgG Class antibodies to Herpes Simplex Virus type 1. | | | Enzyme Immunoassay for the qualitative determination of IgM Class antibodies to Herpes Simplex Virus type 1 & 2. |
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http://www.equipar.it/hsv.htm
(310 words)
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| | WESTERN BLOTS - ZeptoMetrix Corporation |
| | The ZeptoMetrix SIV Western Blot assay is a qualitative enzyme immunoassay for the in vitro detection of antibodies to SIV in serum or plasma. | | | HIV-1 BLOT 1.3 is a qualitative enzyme immunoassay for the detection of antibodies to HIV-1 in human serum or plasma. | | | HTLV BLOT 2.4 is a qualitative enzyme immunoassay for detecting antibodies to HTLV-I and HTLV-II in human serum or plasma. |
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http://www.zeptometrix.com/blots.htm
(348 words)
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| | CiteULike: Tag ferrocene |
| | Synthesis, characterization, and evaluation of ferrocene-theophylline conjugates for use in electrochemical enzyme immunoassay. | | | Multilabeling of ferrocenes to a glucose oxidase-digoxin conjugate for the development of a homogeneous electroenzymatic immunoassay. | | | Electrochemical study of sulphonated ferrocenes as redox mediators in enzyme electrodes |
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http://www.citeulike.org/tag/ferrocene
(587 words)
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| | Serotonin ELISA |
| | BA-10-0900- Enzyme Immunoassay (ELISA) for Serotonin in Urine, serum, PFP,PRP, and CSF | | | Enzyme Immunoassay for the fast and quantitative determination of Serotonin in urine, serum, plasma, and cerebrospinal fluid. | | | License from the NRC/agreement state to handle 125I (RIA's only) |
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http://www.ldn-usa.net/products?cat_no=BA-10-0900
(3627 words)
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| | ARS Publication request: Using Eia to Screen "capsicum" Spp. Germplasm for Capsaicinoid Content |
| | Estimates of pungency as determined by both techniques were in close agreement indicating that the Enzyme Immunoassay may be a suitable and also a less expensive alternative technique for estimating pungency in fresh pepper fruits. | | | Technical Abstract: An enzyme immunoassay (EIA) was evaluated for its ability to quantify total capsaicinoids in fruit of 16 genotypes representing four species of "Capsicum", in comparison with High Performance Liquid Chromatography (HPLC). | | | Lowest concentrations of capsaicinoids occurred in sweet Bell and pimento types ("C. Annum" L.), and the highest concentration occurred in a small-fruited 'bird' type accession of "C. Frutescens" L. Estimates of capsaicinoids obtained by EIA and HPLC were highly correlated (R(2) = 0.996). |
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http://ars.usda.gov/research/publications/publications.htm?SEQ_NO_115=137747
(2366 words)
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| | Auk, The: Corticosterone metabolites can be measured noninvasively in excreta of European Stonechats (Saxicola torquata rubicola) |
| | Therefore a non-invasive technique was developed to measure metabolites of corticosterone in excreta of European Stonechats (Saxicola torquata rubicola) using a corticosterone enzyme-immunoassay. | | | High-performance liquid chromatography of excreta of a female and a male stonechat injected with tritiated corticosterone showed that corticosterone is excreted in the form of numerous metabolites and that the corticosterone enzyme-immunoassay crossreacted with most of those metabolites. | | | They were kept under natural light conditions and housed in individual cages, where they could hear but not see each other. |
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http://www.findarticles.com/p/articles/mi_qa3793/is_200210/ai_n9143192
(1289 words)
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| | Automated immunoassay analyser - Patent 5055408 |
| | The present invention has been made from the standpoint mentioned above, and provides an automated immunoassay analyzer that is suitable to an enzymatic immunoassay system using an enzyme as label in which is measured the amount of change detectable by an optical means that occurs on a substrate as a result of the enzymatic activity. | | | Processes for immunological procedures are different for different labels; thus, they include FIA using a chemical fluorescent material, RIA using a radioactive substance and EIA using an enzyme, each as the label. | | | In the automatic apparatus thus composed, a test plate having a desired number and combinations of test cups mounted on it is first lifted upward by the ascending elevator 5 to the level of the transfer route 7 and then pushed onto the upperstream of the transfer route 7 by the pushing cylinder device 32. |
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http://www.freepatentsonline.com/5055408.html
(4707 words)
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| | Patent 6,573,099 |
| | Wherein the target gene is a gene which is endogenous to the cell, tissue or organ, it is particular preferred that its expression is capable of being monitored by a visual assay, enzyme assay or immunoassay. | | | The synthetic genes described supra are capable of being modified further, for example by the inclusion of marker nucleotide sequences encoding a detectable marker enzyme or a functional analogue or derivative thereof, to facilitate detection of the synthetic gene in a cell, tissue or organ in which it is expressed. | | | As used herein, the term "tyrosinase gene" shall be taken to refer to a structural gene, cDNA molecule, genomic gene or nucleotide sequence which is capable of encoding the tyrosinase enzyme or a polypeptide fragment thereof or alternatively, a nucleotide sequence which is complementary to said structural gene, cDNA molecule, genomic gene or nucleotide sequence. |
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http://pharmcast.com/Patents/Yr2003/Jun2003/060303/6573099_Genetics060303.htm
(4707 words)
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| | GS & AD |
| | The immunoassay detected glutamine synthetase protein in samples where the enzyme had been inactivated by repeated cycles of freezing and thawing, and in serum and cerebrospinal fluid where glutamine synthetase was undetectable by the enzyme activity assay. | | | This protein was identified as glutamine synthetase [GS; glutamate-ammonia ligase; L-glutamate:ammonia ligase (ADP-forming), EC 6.3.1.2] based on similar nucleotide binding properties, comigration on two-dimensional gels, reaction with a polyclonal anti-GS antibody, and the presence of significant GS enzyme activity in AD CSF. | | | Both human and sheep brain glutamine synthetases were composed of three and four different types of subunits with isoelectric points ranging from 7.0-7.2 and 6.8-7.0, respectively. |
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http://www.altcorp.com/AffinityLaboratory/gsad.htm
(2450 words)
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| | page2.html |
| | Beta HCG EIA is a solid phase enzyme immunoassay based on the sandwich principle. | | | These studies on the sensitivities of RIA, ETA and HCG immunoassay (using Gonavislide reagent) have the objective to find a test method for HCG determinathat may offer sound sensitivity coupled with its economic aspect, ie., its relative inexpensiveness. | | | HCG immunoassay on the urine offers a simple, convenient tool for beta HCG analysis. |
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http://www.peoples-research.com/page2.html
(2450 words)
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| | Radioallergosorbent Test (RAST) Methods for Allergen-Specific Immunoglobulin E (IgE) 510(k)s; Final Guidance for Industry and FDA |
| | This generic type of device is intended for use in clinical laboratories and physicians' office laboratories as an in vitro diagnostic test for quantitative, semi-quantitative or qualitative measurement of allergen-specific IgE by various immunological test methodologies such as radioimmunoassay, enzyme immunoassay, chemiluminescent immunoassay, and fluorescent immunoassay. | | | An alternative approach may be used if such approach satisfies the requirements of the applicable statute and regulations. | | | RAST testing measures specific allergen antibodies and may aid in the diagnosis of asthma, allergies, and other pulmonary disorders. |
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http://www.fda.gov/cdrh/ode/guidance/800.html
(4446 words)
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| | Krikounova Vitalia Sergeevna |
| | Krikounova V.S. Development of a polarization fluorescence immunoassay for pesticide 2,4,5-trichlorophenoxyacetic acid, Berezin Foundation Undergraduate and Postgraduate Student International Conference, November 14, 1997, Moscow, Russia. | | | V.S. Krikunova and Sergei A. Eremin Polarization Fluoroimmunoassays of Chlorinated Phenoxyacid Pesticides as Preliminary Screening Method for Dioxins Contamination. | | | Krasnova A.I., Krikunova V., Eremin S.A. Development of an enzyme-linked immunosorbent assay to propanil. |
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http://www.enzyme.chem.msu.ru/eremin/cv-krikunova_www.htm
(4446 words)
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| | SR/MR/Swine - Cypress Diag. |
| | SR/MR SR/MR is an enzyme immunoassay(EIA) for the detection of specific IgG antibodies against Erysipelothrix rhusiopathiae bacterium antigens in pigs sera of vaccinated or convalescent animals of the swine erysipelas disease. | | | In a second step, a Protein A/HRPO conjugate is added to the wells resulting in the specific antibody against Erysipelothrix rhusiopathiae being sandwiched between the solid phase antigen and enzyme conjugate Protein A. After 1 hour incubation at 37°C, the wells are washed with the washing solution to remove unbound material. | | | A substrate/chromogen solution is added and incubated 20 minutes, resulting in the development of a blue color in the wells where a complex Antigen/Anti-Erysipelothrix antibody/Protein A - HRPO conjugate was formed previously. |
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http://www.hlsvbp.com/products/cypress/veterinary/swine/sr_mr.htm
(3232 words)
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| | MSB offers a unpresidented amount of refurbished chemistry products and services. |
| | The ATAC 6000 is a microprocessor controlled chemistry analyzer capable of performing bi-chromatic reading of concentration (end-point) metabolite determinations, kinetic rate measurements of enzyme activity, fixed-time substrate analysis and non-linear enzyme immunoassay. | | | The Hitachi 911 is a fully automated, discrete, computerized chemistry analyzer that uses serum, urine, plasma and CSF sample types to perform in vitro quantitative and qualitative tests on a wide range of alalytes. | | | The Hitachi 705 is a fully automated, discrete, computerized chemistry analyzer intended for in vitro diagnostic use in the quantitative determination of serum constituents. |
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http://www.msb-inc.com/refurb_chemistry.cfm
(1556 words)
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| | homogeneous immunoassay for the detection of trinitrotoluene (tnt) |
| | Proportionality between the TNT concentration and enzyme activity was demonstrated with a detection limit of 5 µg/L TNT. | | | homogeneous immunoassay for the detection of trinitrotoluene (tnt) | | | Homogeneous immunoassay for the detection of trinitrotoluene (TNT) based on reactivation of apoglucose oxidase using a novel FAD-TNT conjugate |
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http://www.ch.tum.de/wasser/weller/FJAC98.htm
(157 words)
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