Elastase - Medicow
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Topic: Elastase



  
 DNase I Acutely Increases Cystic Fibrosis Sputum Elastase Activity and its Potential to Induce Lung Hemorrhage in Mice ...
The potential for rhDNase aerosol therapy to increase sputum elastase levels has recently been studied in CF patients (19).
sputum elastase activity within 1 h of therapy in all CF patients.
Although the effect of rhDNase aerosol therapy on CF sputum elastase activity was transient, it was observed consistently
http://ajrccm.atsjournals.org/cgi/content/full/157/2/464   (3348 words)

  
 Inhibition of Human Neutrophil Elastase-Induced Acute Lung Injury in Hamsters by Recombinant Human Pre-elafin ...
Effect of recombinant human pre-elafin on human neutrophil elastase (HNE)-induced acute lung injury in hamsters.
Barnes, PJ (1998) Chronic obstructive pulmonary disease: new opportunities for drug development.
Functional inhibitory activity of recombinant human pre-elafin and commercial synthetic elafin against human neutrophil elastase.
http://www.chestjournal.org/cgi/content/full/121/2/582   (3223 words)

  
 Leukocyte Elastase . Physiological Functions and Role in Acute Lung Injury -- LEE and DOWNEY 164 (5): 896 -- American ...
These data support the notion that leukocyte elastase is important in the pathogenesis of acute lung injury.
metabolites and neutrophil elastase synergistically cause edematous injury in isolated rat lungs.
-integrins in mediating elastase release is not clear; in
http://ajrccm.atsjournals.org/cgi/content/full/164/5/896   (7029 words)

  
 Neutrophil elastase increases MUC4 expression in normal human bronchial epithelial cells -- Fischer et al. 284 (4): 671 ...
An ultrastructural study of the response of hamster bronchial epithelium to human neutrophil elastase.
Effects of human neutrophil elastase and Pseudomonas aeruginosa proteinases on human respiratory epithelium.
Northern analysis of MUC4 mRNA: concentration-dependent regulation by neutrophil elastase (NE).
http://ajplung.physiology.org/cgi/content/full/284/4/L671   (5206 words)

  
 Synergistic neutrophil elastase-cytokine interaction degrades collagen in three-dimensional culture -- Zhu et al. 281 ...
The effect of cytomix, NE, and the combination was consistently observed in many experiments despite the fact that the actual amount of contraction in any given experiment was somewhat variable.
Elastase did, however, have a clear effect in converting
Values are means ± SE for triplicate gels within a representative experiment.
http://ajplung.physiology.org/cgi/content/full/281/4/L868   (5942 words)

  
 Role of Leukocyte Elastase in Preventing Cellular Re-Colonization of the Mural Thrombus -- Fontaine et al. 164 (6): ...
A: Elastase activity bound to fibrin matrix, preincubated with PMN media (500 µl of conditioned medium corresponding to 5.10
A: Phase contrast photomicrographs of BMSCs in culture.
by addition of natural elastase inhibitors to conditioned media
http://ajp.amjpathol.org/cgi/content/full/164/6/2077   (6078 words)

  
 Elastase-Induced Suppression of Endothelin-Mediated Ca2+ Entry Mechanisms of Vascular Contraction -- Chew et al. 42 ...
to analyze quantitatively the inhibitory effects of elastase.
explain the role of elastase and ET-1 during the development
These studies should help explain the relation between
http://hyper.ahajournals.org/cgi/content/full/42/4/818   (4181 words)

  
 Serum Elastase Activity, Serum Elastase Inhibitors, and Occurrence of Carotid Atherosclerotic Plaques: The Etude sur le ...
Further studies are necessary to elucidate the nature of the
In our study, no association was observed between serum elastase
Several limits of our study should be mentioned.
http://circ.ahajournals.org/cgi/content/full/105/22/2638   (3973 words)

  
 Diet Modifies Elastase I and II Activities and mRNA Levels during Postnatal Development and Weaning in Piglets -- ...
The experimental conditions for quantification of elastase I and
Largman C. Isolation and characterization of rat pancreatic elastase.
Moreover, the elastase I gene is not expressed in the human
http://www.nutrition.org/cgi/content/full/127/11/2205   (4630 words)

  
 Lysophosphatidic acid inhibition of the accumulation of Pseudomonas aeruginosa PAO1 alginate, pyoverdin, elastase and ...
was reduced to 55% of the LB level and elastase was undetectable
Since LB culture supernatants could be diluted 20- to 30-fold
Ojeniji, B. Polyagglutinable Pseudomonas aeruginosa from cystic fibrosis patients: a survey.
http://mic.sgmjournals.org/cgi/content/full/148/6/1709   (7235 words)

  
 Worthington Elastase
Elastase is assayed using a method adapted from that of Feinstein et al., BBRC, 50, 1020, 1973 and using the more soluble substrate of Bieth et al., Biochem.
While elastase will hydrolyze a wide variety of protein substrates, it is unique among proteases in its ability to hydrolyze native elastin, a substrate not attacked by trypsin, chymotrypsin or pepsin.
It is helpful to make primary solutions in KCl or alkaline buffers before diluting into the reaction mixtures or media, compensating for ionic strength or pH changes.
http://www.worthington-biochem.com/ES/cat.html   (262 words)

  
 Elastase
This is truly an example of technology-driven science, when you consider that elastase activity can now be measured under conditions you did not think possible.
This elastase assay works under a variety of conditions.
The measured Km of this substrate with porcine pancreatic elastase is in the 800 nM range, in contrast to the Km values of 6.2 and 0.8 mM for other chromogenic substrates based on pNA and AMC chromophores, respectively [see M.J. Castillo, K.
http://www.phiphilux.com/elas1.htm   (181 words)

  
 Worthington Elastase
(1974) indicate elastase involvement in atherosclerosis and acute hemorrhagic pancreatitis.
Additional information regarding the use of elastase in tissue dissociation can be found in the Worthington Tissue Dissociation Guide.
Elastase is distinctive in that it acts upon elastin.
http://www.worthington-biochem.com/ES   (2330 words)

  
 Presence of the M-type sPLA2 receptor on neutrophils and its role in elastase release and adhesion -- Silliman et al. ...
Previous studies from our laboratory demonstrated that sPLA
pathways for elastase release in the PMN that involve the activation
cellular elastase as determined by 0.1% Triton-X paired treatment
http://ajpcell.physiology.org/cgi/content/full/283/4/C1102   (6778 words)

  
 Nitric oxide reduces vascular smooth muscle cell elastase activity through cGMP-mediated suppression of ERK ...
Our previous studies showed that STE-induced elastase activity
This is a representative gel of three independent experiments with similar results.
elastase activity induced in cultured vascular smooth muscle
http://www.fasebj.org/cgi/content/full/14/5/805   (6490 words)

  
 Elastase - Wikipedia, the free encyclopedia
Neutrophil elastase is responsible for the blistering in bullous pemphigoid, a skin condition, in the presence of antibodies.
Elastase is inhibited by the acute phase protein α
There appear to be two forms of neutrophil elastase, termed IIa and IIb.
http://en.wikipedia.org/wiki/Elastase   (295 words)

  
 Emphysema - Wikipedia, the free encyclopedia
This hypothesis, a result of the observation that NE is the primary substrate for A1AT, and A1AT is the primary inhibitor of NE, together have been known as the "protease-antiprotease" theory, implicating neutrophils as an important mediator of the disease.
Most A1AD patients do not develop clinically significant emphysema, but smoking and severely decreased A1AT levels (10-15%) can cause emphysema at a young age.
Studies for the better part of the past century have focused primarily upon the putative role of leukocyte elastase (also neutrophil elastase), a serine protease found in neutrophils, as a primary contributor to the connective tissue damage seen in the disease.
http://en.wikipedia.org/wiki/Emphysema   (1114 words)

  
 Elastase definition - Medical Dictionary definitions of popular medical terms
Chronic Obstructive Pulmonary Disease - Learn about COPD - Chronic Obstructive Pulmonary Disease which is primarily two related diseases - chronic bronchitis and emphysema.
Elastase: An enzyme that digests and degrades a number of proteins including elastin, an elastic substance in the lungs and some other organs that supports their structural framework.
Elastase definition - Medical Dictionary definitions of popular medical terms
http://www.medterms.com/script/main/art.asp?articlekey=33860   (175 words)

  
 Fecal elastase - Wikipedia, the free encyclopedia
Role of Fecal Elastase 1 in Pancreatic Cancer: A Pilot Study [2]
Fecal elastase refers to the testing of the concentration of the pancreatic elastase-1 enzyme found in fecal matter with an enzyme-linked immunosorbent assay (ELISA).
This page was last modified 01:43, 2 March 2005.
http://en.wikipedia.org/wiki/Fecal_elastase   (142 words)

  
 Microscopes - Fluorescent Microscope ☼
Sony technolook,Scalar video microscopes combine electronic video cameras, special lighting, and powerful magnifying lenses to vividly show highly enlarged moving images
Detect apoptosis, elastase, inflammation, metastasis, viruses, capsase, CPP32, ICE
http://www.microscopes-online.info/fluorescentmicroscope   (1746 words)

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