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| | AID EliSpot READER SYSTEM - Homepage |
 | | The ELISPOT assay is a specific, sensitive, quantitative assay for assessing cell-mediated immune responses to a variety of antigens including HIV-1 peptides. | | | The importance of the enzyme-linked immunosorbent spot (ELISPOT) assay as a tool for studying immune responses in vitro is becoming increasingly apparent. | | | The classic AID EliSpot Reader System has been the choice of many distinguished research labs on all continents. |
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http://www.elispot.com/method.htm
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| | Globeinvestor.com: SeraCare Life Sciences and Cellular Technology Announce Collaboration |
| | The ELISPOT technology allows for in-vitro determination of the effectiveness of different "development candidates" because not only can total cytokine response be measured, but the response of individual cytokines can also be quantified. | | | The new, joint products are expected to facilitate the adaptation of ELISPOT and related technologies to clinical immune diagnostics, and to accelerate basic research. | | | The widespread acceptance of the ELISPOT platform has led to CTL's leadership position in immune diagnostics. |
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http://www.globeinvestor.com/servlet/WireFeedRedirect?cf=GlobeInvestor/config&vg=BigAdVariableGenerator&date=20041207&archive=bwire&slug=20041207005153
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| | KPL ELISpot Kits and Reagents |
| | Based on the traditional and commonplace ELISA technique, ELISpot protocols use many common reagents and steps associated with the original microwell assay. | | | The high specificity and sensitivity of the ELISpot assay makes this an ideal procedure compared to traditional quantitative methodologies. | | | The ELISpot (Enzyme Linked Immuno-Spot) assay is powerful method used for the detection of individual cells secreting a protein or cytokine of interest. |
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http://www.kpl.com/catalog/categories.cfm?Catalog_ID=2&Category_ID=355
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| | ITN - Core Facilitiies |
| | Principal Investigators determine the selection of cytokines and antigens to be used in the ELISPOT assay in conjunction with the Core and Tolerance Assay Directors. | | | In addition, the facility can use peptides derived from the polymorphic regions of donor HLA molecules to assess reactivity through the indirect pathway. | | | The ITN ELISPOT Core provides analyses of frozen cell samples from patient peripheral blood for secreted cytokine assays. |
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http://www.immunetolerance.org/research/core/facilities/elispot.html
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| | CCR Frontiers in Science May 2005 Volume 4 |
| | All ELISPOT assays are subjected to automated analysis using the Immunospot Analyzer from Cellular Technology, Ltd. The laboratory continues to tailor other current methods as well as develop new procedures to assess immune responses in clinical trials. | | | All assays have been approved for use by the Vaccine Working Group Steering Committee (Chair Dr. Jay Berzofsky) and are available to NIH researchers. | | | A whole-protein IFN-γ ELISPOT was developed by LCMI to monitor immune responses to vaccinations with whole proteins or peptides greater than 10 mer. |
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http://ccr.nci.nih.gov/news/frontiers/may2005/biotech_resources_1.html
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| | Spotlight for April 05 |
| | The data obtained from this assay help determine whether a new vaccine has been successful in strengthening the patient’s immune response. | | | The LCMI team has adapted this assay specifically for monitoring cancer vaccine trials. | | | Combining these assays helps the clinical investigator perform more comprehensive immunological monitoring of patients participating in cancer vaccine trials. |
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http://www.ncifcrf.gov/news/spotlight/sl_april05.asp
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| | [No title] |
| | This meeting is for anyone who is using or thinking about using the ELISPOT technique | | | There will be plenty of opportunities to discuss the suitability of these techniques for your individual research together with any technical problems you might be having | | | Attendees are invited to bring their ELISPOT plates for analysis on site during the breaks by Sanquin reagents |
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http://www.euroscicon.com/elispot.html
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| | ELISPOT |
| | Nothing much to say today, but in the interest of cross-culturalism, I thought I'd post links to the German WikiPedia ELISPOT page. | | | Time hasn't allowed me to maintain this on-line diary as much as I'd like, but I did want to mention something about this. | | | Here is an animated conceptualization of the ELISA process, the predecessor to the ELISPOT technique. |
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http://elispot.blogspot.com
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| | ELISPOT - Simple English Wikipedia |
| | Examples of spot patterns formed by the ELISPOT technique | | | The ELISPOT method is typically used with blood cells, and is a helpful tool in studying the human immune system and various treatments for disease (e.g. | | | The ELISPOT technique is very sensitive, and can detect even a single substance-producing cell. |
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http://simple.wikipedia.org/wiki/ELISPOT
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| | Millipore Catalogue - MultiScreen<sub>HTS</sub> Filter Plates for Elispot |
| | Elispot assays are used for a range of applications to monitor immune responses and immunological response patterns, and can also be used to identify antigenic peptides and create vaccination strategies. | | | Filter Plates for Elispot,The ELISPOT assay makes it possible to visualize single cells that recognize a specific structure of interest, i.e., an antigen. | | | The wells were seeded with 50,000 cells and developed using BCIP/NBTplus substrate. |
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http://www.millipore.com/catalogue.nsf/docs/C9039
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| | 223-T. A Systematic Comparison of Methods to Measure HIV-1-Specific CD8 T Cells |
| | CRA and ELISPOT assays using Rvv did not differ regarding their capacity to detect HIV-specific CTL responses despite some divergences observed in progressors. | | | These observations were observed both in LTNP and progressors. | | | Third, to compare the memory CTL analysis using CRA and the effector CD8 cell analysis using ex-vivo ELISPOT assay. |
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http://www.retroconference.org/2002/Abstract/13538.htm
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| | Scanalytics: Press Release 8/23/99 |
| | Certain aspects of immunological research focus on the role of lymphocytes in the human immune response, specifically, with cell-cell communication via cytokine molecules. | | | Six-Month Study to Refine IPLab Elispot Imager for Use in Immunological Research Studies | | | Elispot assays were originally developed to detect individual antibody-secreting and cytokine-secreting lymphocyte cells. |
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http://www.scanalytics.com/info/archives/PressRel/pr99/pr9908a.shtml
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| | Granzyme B ELISPOT assay for ex vivo measurements of T cell immunity. |
| | We established granzyme B (grB) enzyme-linked immunospot assay (ELISPOT) in conjunction with image analysis to this end. | | | Reactivity to HIV peptides was seen in grB ELISPOT assays of freshly isolated PBMC from HIV patients, consistent with the detection of peptide-specific memory cells. | | | The state of the art may have changed since the publication date. |
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http://www.aegis.com/aidsline/2000/oct/A00A0971.html
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| | Elispot plates column |
| | The second set of experiments focused on the effect of pre-wetting with 15µL of 70% ethanol on Elispot assay performance. | | | The major difference between NC and PVDF in ELISPOT applications is related to their mechanisms of binding and associated differences in handling or pretreatment. | | | Visiting this web site without first upgrading your browser may result in unreliable behavior. |
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http://www.zellnet.com/elispotplatescolumn
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| | Cancer Immunity 4:12 (2004) |
| | For all experiments, influenza nucleoprotein 206-229 peptide, previously shown to be immunogenic in the majority of individuals (18), was used as a positive control. | | | In contrast, only 3/14 (21%) HLA-DP4+, NY-ESO-1 antibody-negative patients (patients 7, 23, and 24) demonstrated a CD4+ T cell response by IFN-gamma ELISPOT. | | | The specificity of the IFN-gamma ELISPOT assay was confirmed by demonstrating that CD4+ T cells that were reactive against influenza nucleoprotein peptide 206-229 from patients did not produce IFN-gamma in response to the irrelevant DP4 157-170 peptide. |
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http://www.cancerimmunity.org/v4p12/040914.htm
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| | Scanalytics: Press Release 6/14/99 |
| | IPLab Elispot Imager is a fully integrated, dual-platform imaging and analysis workstation. | | | This user-configurable system can scan and analyze a plate in minutes, and contains analytical parameters which can be scripted for run-to-run reproducibility. | | | System Components include dual illumination fiber optics; a magnifying close-up lens with side-arm fiber optic illumination; a high-precision X-Y stage; a high-quality camera stand; and a joystick for setting up different varieties of plates and analyzing individually selected wells. |
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http://www.scanalytics.com/info/archives/PressRel/pr99/pr9906a.shtml
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| | 212-T. Correlation between Frequencies of HIV-1-p24-Specific Th1 Cells (ELISPOT) and Plasma HIV-1 Viral Load in a ... |
| | The HIV-1-p24-specific T-cell responses were analyzed using 3 methods: ELISPOT assay for single-cell IFN- | | | Background : HIV-1-specific T helper-1 cell responses have been associated with long-term-non-progression (LT-NP) in HIV infection but the correlation between frequencies of HIV-1-p24 specific Th1 cells and viral load have not yet been studied. | | | Results : Wide ranges were observed in the frequencies of HIV-1-p24-specific CD4 Th1 cells as assessed by ELISPOT (0-3770 SFC/10 |
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http://www.retroconference.org/2002/Abstract/13447.htm
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| | Table of contents for Handbook of ELISPOT methods and protocols |
| | ELISPOT ASSAY IN ANIMAL MODELS 9 Improving the Sensitivity of the ELISPOT Analyses of Antigen-Specific Cellular Immune Responses in Rhesus Macaques K. | | | Contents may have variations from the printed book or be incomplete or contain other coding. | | | Table of contents for Handbook of ELISPOT methods and protocols |
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http://www.loc.gov/catdir/toc/ecip0420/2004016928.html
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| | Millipore - Technical Library - IFN-y ELISpot Assays on MultiScreen IP |
| | The popularity of this assay has seen resurgence in recent years as researchers attempt to gain a better understanding of immune responses in a variety of applications. | | | It may be optimized as necessary for other applications. | | | The Elispot (Enzyme Linked Immuno-Spot) assay provides an effective method of measuring the antibody or cytokine production of immune cells on the single cell level. |
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http://www.millipore.com/publications.nsf/f2131505cb602a2f852568b7005940b0/bb31062221cb3f4e85256999004833dc?OpenDocument
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| | Endpoint Assays in Support of HIV Vaccine Trials: Update and Plans |
| | Publish a summary of this workshop as guidance to individuals using ELIspot assays in clinical trials. | | | To define common elements of a laboratory program for ELISPOT QA: | | | (2) Discussing scientific and operational issues associated with ELISPOT assay performance and attempt to identify the variables or "sources of noise" based on the PT panel data. |
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http://www.niaid.nih.gov/daids/vaccine/studies/endpoint.htm
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| | mabtech.com Mabtech AB |
| | View our list of reference articles regarding ELISA and ELISpot. | | | is a Swedish biotechnology company that has been at the forefront of the ELISpot technique for more than a decade. | | | Mabtech offers ELISA and ELISpot reagents for Human, Monkey, Mouse and Bovine cytokines. |
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http://www.mabtech.com
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| | Cytokine ELISPOT assays, analyzers and robotics |
| | The ELISPOT assay was originally developed for analyzing specific antibody-secreting cells; however, it has been adapted for enumerating cells which secrete other effector molecules, such as cytokines, chemokines and granzymes. | | | This avoids most of the tedium involved in this process, and if scientifically validated statistical techniques are used, the subjectivity involved can be eliminated as well. | | | The high sensitivity and flexibility of this technique are among the attractive features of the ELISPOT assay method. |
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http://dora.cwru.edu/vbv/cytokine-elispot-assays.html
(411 words)
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| | Morphology - Open Encyclopedia |
| | In digital image processing, morphology is a collection of techniques based on Mathematical Morphology, a theoretical model based on Lattice theory. | | | In biology, morphology is the study of the form or shape of an organism or part thereof. | | | It can also be used to describe the form or shape of patterns formed in the course of a biological assay, as with bacterial colonies or ELISPOT assay spot formations. |
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http://open-encyclopedia.com/Morphology
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| | ELISpot Kits |
| | A basic understanding of ELISpot assay development is required for the successful use of these reagents. | | | Each investigator should optimize the experimental conditions, such as cell type, cell stimulation conditions and cell dilutions for each assay. | | | Complete ELISpot kits are ready-to-run and require no assay development or refinement. |
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http://www.rndsystems.com/asp/g_sitebuilder.asp?BodyId=496
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| | Immunology and cytokine ELISPOT assays |
| | While ELISPOT assay techniques have existed for two decades now, modern ELISPOT analysis is typically performed using ELISPOT readers, which employ computer vision techniques to enumerate the cytokine-producing cells. | | | This allows much of the analysis process to be automated, and permits a greater level of accuracy than what can be achieved using manual inspection. | | | One such tool is the cytokine ELISPOT assay, which provides a means to count cytokine-producing cells within a suspension. |
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http://www.angelfire.com/mech/elispot/cytokine-elispot.html
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| | Products - ELISPOT Starter Kit |
| | ELISPOTs are enzyme-linked immunospot assays for the analysis of specific cytokine production at the single cell level. | | | The ELISPOT is now commonly used to measure the number of individual T cells that are secreting a specific cytokine after stimulation with a specific antigen or peptide. | | | Originally, the ELISPOT technique was used to enumerate B cells that were secreting antibodies. |
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http://www.piercenet.com/Products/Browse.cfm?fldID=20A5A7AD-9F2F-4980-A337-96AEEA181DEF
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| | Cytokine Expression Profile, ELISPOT |
| | However, the experimental conditions including the number of cells, the best combination of antibodies, and the stimulation and incubation times for optimal cytokine release have to be empirically determined. | | | ELISPOT (Enzyme-linked ImmunoSPOT) technique was originally used to enumerate antibody secreting B cells. | | | Due to experimental variation, tweaking of the number of cells depositied per well, the proper stimulation and culture period for secretion of a specific cytokine may be required. |
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http://www.ebioscience.com/ebioscience/appls/ELISPOT.htm
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| | The Immune System; an Introduction to Immunology |
| | Lately, I've been studying immunology -- partly out of interest, and partly because this background will prove helpful in some work that I'm doing. | | | This includes reading about cellular immunology in general, and also about the ELISPOT process. | | | Soluble polypeptides (hormones) which regulate the immune response by mediating cellular interactions and regulating cell growth and function. |
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http://www.geocities.com/johnnyt84047/elispot-immunology.html
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| | Immunology, ELISPOT and more |
| | I have a subpage which say something on cellular immunology and the ELISPOT process, but it needs to be threshed out more. | | | I'll say something about cytokines, and antibodies, and interferon, and maybe discuss ELISPOT analysis in greater detail. | | | I need to add more substance to it, after I've composed more information. |
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http://www.angelfire.com/mech/elispot
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| | Zellnet Consulting - The Art of the Elispot Technique |
| | Zellnet Consulting - The Art of the Elispot Technique | | | ZellNet Consulting - The Art of the Elispot Technique | | | Visiting this web site without first upgrading your browser may result in unreliable behavior. |
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http://www.zellnet.com
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| | BD Biosciences |
| | Our BD™ ELISPOT Kits with pre-coated plates, sets, and pairs are available for human, non-human primate, and mouse research. | | | Although originally developed for analyzing specific antibody-secreting cells, the assay has been adapted for measuring the frequencies of cells that produce and secrete a variety of other effector molecules such as cytokines. | | | The enzyme-linked immunospot (ELISPOT) assay is a powerful tool for detecting and analyzing individual cells that secrete a particular protein in vitro. |
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http://www.bdbiosciences.com/pharmingen/products/display_product.php?keyID=19
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| | Immunohistology Kits and Reagents for Tissue Staining and ELISpot |
| | Immunohistochemistry techniques utilize an antibody to probe and visualize cellular antigens in situ, by chromogenic or fluorescent methods. | | | For greatest sensitivity, a biotin-streptavidin system should be used. | | | Another related technique, the ELISpot (Enzyme Linked ImmunoSpot) assay also provides a means of detecting antigens or antibodies in single cells on a solid support. |
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http://www.kpl.com/catalog/categories.cfm?Catalog_ID=2&Category_ID=189
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| | BIONOR ELISPOT |
| | Kit for detection of the respective cytokine producing cells in human Peripheral Blood Lymphocytes (PBLs). | | | BIONOR ELISPOT is available for the following cytokines: |
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http://www.bionor.no/elispot.html
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| | KS EliSpot for biomedical imaging |
| | KS ELISPOT provides high-resolution evaluation of Elispot assays in applications such as Interferon- g and TNF-a where spot diameters are less than 25µm. | | | Elispot (Enzyme Linked Immuno Spot) evaluation is a sensitive method for detecting and quantifying individual T- lymphocytes which form cytokine spots following antigen contact in vitro | | | For applications such as TNF-a where very high resolution is required it is possible to upgrade to KS ELISPOT |
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http://www.imas.co.uk/pages/biomedical_img/elispot/elispot.html
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