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| | The Utility of DNA Microarrays for Characterizing Genotoxicity |
 | | Other important components of our evaluation were to ensure that one could distinguish a genotoxicity profile from a cytotoxicity profile and to evaluate the value of the technology for examining low-dose effects of genotoxic agents. | | | A high concentration that induced 70-80% cytotoxicity (for a TK gene mutation assay) or approximately 50% cytotoxicity (for a micronucleus assay) with a robust induction of genotoxicity and a low concentration that produced limited cytotoxicity (10-30%) with a 1.5- to 4-fold increase in genotoxicity (gene mutations, chromosomal damage, or DNA adducts) were used. | | | For the Genotoxicity Working Group, as well as for other investigators in the field of toxicogenomics, a significant challenge has been the variation in approaches to data presentation and analysis across laboratories. |
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http://ehp.niehs.nih.gov/txg/members/2004/6709/6709.html?...
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| | Epidemiologic Notes and Reports Cytotoxicity of Volcanic Ash: Assessing the Risk for Pneumoconiosis |
| | Assays for cytotoxicity were based on the hemolysis of the red cells of sheep and the release of the enzymes, lactate dehydrogenase, B-N-acetylglucosaminidase, and B-glucuronidase, from alveolar macrophages (Table 3). | | | However, the sample of volcanic ash from Galunggung was significantly more cytotoxic than the other ash samples and approximated the cytotoxicity of quartz. | | | To determine whether these results are representative of other types of volcanic ash, NIOSH compared the cytotoxicity of samples from the eruptions of Mount St. Helens, El Chichon, and Galunggung with those of a mineral of known cytotoxicity, quartz, and a relatively inert mineral, barite. |
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http://www.cdc.gov/mmwr/preview/mmwrhtml/00000724.htm
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| | Dr. Manoj Monga's CV Printout |
| | Shiozawa H, Monga M, Rajasekaran M: Characterization of hydrogen peroxide induced cytotoxicity to rabbit cavernosal smooth muscle cells in culture. | | | Shiozawa H, Rajasekaran M, Monga M: Cavernosal cell cytotoxicity: Development of a cell culture model. | | | Rajasekaran M, Shiozawa H, Monga M: Characterization of hydrogen peroxide induced cytotoxicity to rabbit cavernosal smooth muscle cells in culture. |
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http://www.urotrials.umn.edu/mdpages/monga/cv/MongaPrint.htm
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| | NK FUNCTION IN FLOW CYTOMETRY |
| | NK cells can kill their targets by different mechanisms: i) cell-mediated: this can take place through the classical, perforin-mediated target membrane damage, or through the induction of granzyme-mediated cytotoxicity. | | | The flow cytometry methodologies that follow describe: i) the measurement of NK binding to targets; ii) the measurement of NK cytotoxicity by propidium iodide staining of dead targets; iii) the contemporary measurement of binding and cytotoxicity, which gives the percentage of target cells effectively killed by the NK cells bound on their surface. | | | It has numerous advantages upon other techniques which can measure NK binding and cytotoxicity. |
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http://www.cyto.purdue.edu/flowcyt/research/cytotech/amfc/data/page12.htm
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| | Genotoxicity Cytotoxicity Bioassay Mutagenicity Biocompatibility Mikrobiologie Skin Toxicity Medical Device Testing Notification Google |
| | genotoxicity, cytotoxicity, bioassay, mutagenicity, biocompatibility, mikrobiologie, skin, bioservice, toxicity, medical, device, testing, | | | Genotoxicity Cytotoxicity Bioassay Mutagenicity Biocompatibility Mikrobiologie Skin Toxicity Medical Device Testing Notification Google | | | Medical, Device, Testing, Genotoxicity, Notification, Legionellen, Cosmetics, Genotoxicity, Cytotoxicity, Bioassay, Toxicity, GMP |
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http://www.bslbioservice.de
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| | 1997 July 22, sci.med.aids thread - Licorice and Cell mediated cytotoxicity |
| | These results suggest that the decrease of elevated transaminase levels by glycyrrhizin in patients with chronic viral hepatitis is mediated in part by inhibition of immune-mediated cytotoxicity against hepatocytes. | | | Intravenous administration of glycyrrhizin is known to decrease elevated plasma transaminase levels in patients with chronic viral hepatitis, in which immune-mediated cytotoxicity by cytotoxic T lymphocytes and tumour necrosis factor (TNF)-alpha is considered to play an important pathogenic role. | | | Glycyrrhizin inhibited the cytotoxic activity of the T cells against antigen-presenting cells and also suppressed TNF-alpha-induced cytotoxicity in the TNF-alpha-sensitive cell line in vitro. |
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http://www.hsph.harvard.edu/Organizations/healthnet/HIV/docs/scimed02/SciMed01520.html
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| | showme.asp?id=53 |
| | Brahmi, Z., Bochan, M., Su, B., Montel, A. and Morse, P.: Synergistic inhibition of human cell-mediated cytotoxicity by complement component antisera indicates that target cell lysis may result from an enzymatic cascade involving granzymes and perforin. | | | Bajpai, A. and Brahmi, Z.: Regulation of natural killer cell-mediated cytotoxicity by serine/threonine phosphatases: Identification of a calyculin A sensitive serine/threonine kinase. | | | Montel, A., Bochan, M., Hobbs, J., Lynch, D. and Brahmi, Z.: Fas involvement in cytotoxicity mediated by human NK cells. |
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http://medicine.iupui.edu/faculty/showme.asp?id=53
(665 words)
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| | In vitro investigation of uranium cytotoxicity after acute exposure of NRK-52E rat renal proximal tubule epithelial cells |
| | Classical methylthiazol tetrazolium (MTT), lactate dehydrogenase (LDH) and sulforhodamine B tests were used to determine CI50 cytotoxicity indexes, uranium concentration leading to 50% cell death, of these two uranium formulations. | | | Our results show that uranyl complexes cytotoxicity highly depends on the counter ion, and that cytotoxicity is the same, as measured with the three biochemical tests. | | | Toxicity after 24 h exposure was evaluated using MTT (squares), sulforhodamine B (triangles) or lactate dehydrogenase (circles) biochemical tests. |
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http://www-drecam.cea.fr/en/Phocea/Vie_des_labos/Ast/ast_visu.php?id_ast=430&&print=1
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| | Cancer Spectrum: Wang et al., pp. 1473-1478. |
| | cytotoxicity of melphalan, which is used in the treatment of | | | The cytotoxicity of melphalan, a nitrogen mustard analogue (Sigma | | | Linear regression analysis of melphalan cytotoxicity versus the relative increase in the density of Rad51 foci in eight tumor cell lines treated with 5.5 µM melphalan for 4 hours. |
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http://jncicancerspectrum.oxfordjournals.org/cgi/content/full/jnci;93/19/1473
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| | Standardized cytotoxicity tests for dental composite materials with quality assurance |
| | Conclusion: Cytotoxicity tests should be further standardized, particularly in regard to specimen production protocols, to ensure results are internationally comparable and to make these tests useful as a tool for risk assessment. | | | Standardized cytotoxicity tests for dental composite materials with quality assurance | | | (3) The cytotoxicity of each composite was significantly higher when polymerized in 5-mm than 2.5-mm increments (p<0.0001). |
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http://iadr.confex.com/iadr/2004Hawaii/techprogram/abstract_44761.htm
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| | In Vitro Cytotoxicity Assays of Human Epidermal Keratinocytes in Culture Exposed to Sulfur Mustard - Storming Media |
| | Our laboratory has reported problems associated with simple vital dye uptake as a measure of cytotoxicity in human epidermal keratinocytes (HEK) following in vitro exposure to HD. | | | In Vitro Cytotoxicity Assays of Human Epidermal Keratinocytes in Culture Exposed to Sulfur Mustard | | | Abstract: A number of studies have described several in vitro cytotoxicity assays that are convenient methods for assessing cell viability. |
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http://www.stormingmedia.us/38/3815/A381583.html
(4328 words)
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| | Genotoxicity Cytotoxicity Bioassay Mutagenicity Biocompatibility Mikrobiologie Skin Toxicity Medical Device Testing Notification Google |
| | genotoxicity, cytotoxicity, bioassay, mutagenicity, biocompatibility, mikrobiologie, skin, bioservice, toxicity, medical, device, testing, | | | cytotoxicity, bioassay, mutagenicity, biocompatibility, mikrobiologie, skin, bioservice, toxicity, medical, device, testing, notification, | | | Genotoxicity Cytotoxicity Bioassay Mutagenicity Biocompatibility Mikrobiologie Skin Toxicity Medical Device Testing Notification Google |
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http://www.bslbioservice.de
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| | Multiple Forms of Angiostatin Induce Apoptosis in Endothelial Cells -- Lucas et al. 92 (12): 4730 -- Blood |
| | Cytotoxicity was assessed using the ethidium homodimer incorporation assay, and results are shown as the mean of duplicate samples. | | | At the end of the incubation, endothelial cell cytotoxicity was assessed using the ethidium homodimer incorporation assay. | | | Confluent monolayers of BME, DMVE, or rat aortic smooth muscle (RASM) cells were treated with ehAst or LPS at the indicated concentrations, and endothelial cell cytotoxicity was assessed using the ethidium homodimer incorporation assay after 72 hours. |
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http://www.bloodjournal.org/cgi/content/full/92/12/4730
(6591 words)
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| | Energy Citations Database (ECD) - Energy and Energy-Related Bibliographic Citations |
| | Although there is an association between survival, antiport function, and pH{sub i} level under most treatment conditions, the precise role of the Na{sup+}/H{sup+} antiport in mediating thermal cytotoxicity remains uncertain. | | | Conclusion: We demonstrated that damage to the Na{sup+}/H{sup+} antiport likely reflects early heat-induced change in membrane function, but is not a primary target for heat cytotoxicity. | | | When the same degree of cytotoxicity was induced by ionizing radiation, no alteration in Na{sup+}/H{sup+} antiport function was observed. |
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http://www.osti.gov/energycitations/product.biblio.jsp?osti_id=20422928
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| | Cancer Spectrum: Medline Abstract |
| | Cleavable complex formation and cytotoxicity induced by CPT and SN-38 correlate with topo I level and activity in cells at different stages in their differentiation. | | | In general, cytotoxicity closely paralleled cleavable complex formation, as attested to by the four- to 6-fold decrease in cytotoxicity in fully differentiated cells treated with CPT and SN-38 compared with proliferating cells. | | | We show that cleavable complex formation in vivo (on genomic DNA) induced by CPT or SN-38 is 4- to 7-fold reduced in fully differentiated cells relative to undifferentiated cells. |
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http://jncicancerspectrum.oxfordjournals.org/cgi/medline/pmid;11668498
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| | 1997, sci.med.aids thread - FLT & Too Much TP |
| | The levels of nucleoside analog triphosphate shown here [Table 1] correlate directly with the cytotoxicities of these analogs measured previously in CEM cells and suggest that the relatively high cytotoxicity of FLT may be due to its anabolism with an efficiency apparently indistinguishable from that of thymidine. | | | They state "The levels of nucleoside analog triphosphate shown here [Table 1] correlate directly with the cytotoxicities of these analogs measured previously in CEM cells and suggest that the relatively high cytotoxicity of FLT may be due to its anabolism with an efficiency apparently indistinguishable from that of thymidine." | | | However, the same triphosphate can also serve as a substrate for host DNA polymerases, can be incorporated into the DNA of the host, and can cause cytotoxicity. |
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http://www.hsph.harvard.edu/Organizations/healthnet/HIV/docs/scimed02/SciMed01540.html
(488 words)
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| | S096808960000221.abstract.en |
| | In this article, we report synthesis and cytotoxicity of 4´-C- and 5´-C-substituted toyocamycins, which were prepared via the condensations of 4-C- and 5-C-substituted ribofuranose derivatives 11, 12, 13, 20, 21, and 26 with the silylated form of 4-amino-6-bromo-5-cyanopyrrolo[2,3-d]pyrimidine (27) and subsequent debromination and debenzoylation. | | | Toyocamycin and some analogues have shown potent antitumor activities; however, none of them could be used clinically primarily owing to their cytotoxicity to normal human cells. | | | When compared to the parent toyocamycin, all these analogues showed much lower cytotoxicity to human prostate cancer cells (HTB-81), mouse melanoma cancer cells (B16) as well as normal human fibroblasts. |
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http://www.elsevier.com/cdweb/journals/09680896/articles/9/1/S096808960000221.abstract.en
(173 words)
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| | CaP CURE, Association for the Cure of Cancer of the Prostate |
| | Prostate cancer usually express GnRH receptors and therefore these GnRH conjugates have a potential chemotherapeutic advantage since cytotoxicity is restricted to the illuminated area. | | | The cytotoxicity could be demonstrated by the addition of a GnRH analog which competed for the same binding sites, thus deminished that the cytotoxicity is receptor mediated. | | | Upon illumination, photosensitizers generate cytotoxic singlet molecular oxygen. |
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http://www.capcure.org.il/summary7.htm
(173 words)
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| | (ISO 10993) A Practical Guide to ISO 10993-5: Cytotoxicity (MDDI archive, Apr 98) |
| | Cytotoxicity test methods are useful for screening materials that may be used in medical devices because they serve to separate reactive from nonreactive materials, providing predictive evidence of material biocompatibility. | | | Required for all types of devices, cellular toxicity testing is covered in 10993-5: "Tests for Cytotoxicity—In Vitro Methods." This standard presents a number of test methods designed to evaluate the acute adverse biological effects of extractables from medical device materials. | | | Cytotoxicity test methods are also useful for lot-to-lot comparison of materials, for determining whether a potential replacement material is equivalent to that currently being used, and for troubleshooting and exploring the significance of changes in manufacturing processes. |
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http://www.devicelink.com/mddi/archive/98/04/013.html
(1182 words)
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| | Keith Koistinen, 2001 Merck-Merial Summer Scholar |
| | The non-enzymatic browning reaction proved to reduce the cytotoxicity of fumonisin B1 (FB The FB was also detoxified when stored at freezing temperatures for several months with Dulbecco's Modified Eagles Medium (DMEM) and with DMEM and dimethyl sulfoxide (DMSO). | | | The nonenzymatic browning reaction was found to reduce the cytotoxicity of FB. | | | The non-enzymatic browning reaction by the extrusion of corn could prove to be a possible method to detoxify fumonisin-contaminated corn. |
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http://www.vetmed.iastate.edu/research/summerscholars/past/merck/2001participants/koistinen.asp
(571 words)
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| | 20040822_Stanford_Content.txt |
| | Revised parentage: is-a to "immune cell mediated cytotoxicity," above, and is-a to GO:00069174 induction of apoptosis Comment: Note that either or both mechanisms mentioned in the definition may be used in this process. | | | Reference: ISBN:0781735149 9) GO:0042267 natural killer cell mediated cytolysis This term needs to be renamed and redefined to parallel the T-cell mediated cytotoxicity term, because the mechanisms of cell killing used by NK cells are nearly identical to those of T cells, and involve the induction of apoptosis in the target cells. | | | Autolysis should be an is-a child of cytolysis. |
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http://www.geneontology.org/minutes/20040822_Stanford_Content/20040822_Stanford_Content.txt
(9386 words)
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| | Echinacea - RxList Alternatives |
| | Broumand N, et al.: In Vitro Effects of Echinacea and Ginseng on Natural Killer and Antibody-Dependent Cell Cytotoxicity in Healthy Subjects and Chronic Fatigue Syndrome or Acquired Immunodeficiency Syndrome Patients., Immunopharmacology, 1997, 35(3):229-235. | | | DM Broumand N, Sahl L, Tilles JG: In Vitro Effects of Echinacea and Ginseng on Natural Killer and Antibody-dependent Cell Cytotoxicity in Healthy Subjects and Chronic Fatigue syndrome or Acquired Immunodeficiency Syndrome Patients., Immunopharmacology, 1997, Jan, 35(3):229-35ISSN: 0162-3109 | | | Elsasser-Beile U, Willenbacher W, Bartsch HH, Gallati H, Schulte Monting J, vonKleist S: Cytokine production in Leukocyte Cultures During Therapy with Echinacea extract., J Clin Lab Anal, 1996, 10(6):441-5 ISSN: 0887-8013 |
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http://www.rxlist.com/cgi/alt/echinacea_ids.htm
(9386 words)
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| | Natürlich gegen Krebs Erfahrungsberichte |
| | The influences of in vivo treatment with two pure PCB congeners, 2,2', 4, 4 ',5,5'-hexachlorobiphenyl (HCBP) and 3, 3 ', 4, 4 '-tetrachlorobiphenyl (TCBP), on the lethal cytotoxicity of bromobenzene and acetaminophen were examined in short-term primary cultures of isolated rat hepatocytes. | | | TCBP and 3 -MC each increased (20- to 30-fold) cytotoxicity of acetaminophen by a mechanism substantially inhibitable by ANF but not SKF-525- A. | | | The HCBP, a PB-type inducer of cytochrome P-450, resembled phenobarbitone (PB) in its ability to increase susceptibility of hepatocytes to bromobenzene (0.5 to 1.6 mM) and acetaminophen (1 to 16 mM). |
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http://www.stopping-cancer-naturally.org/uk/rettet_dominik/studien_usa/comparative_influences_of_different.html
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