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 Apolipoprotein A-I Induces Translocation of Cholesterol, Phospholipid, and Caveolin-1 to Cytosol in Rat Astrocytes -- ...
Synthesis and translocation to the cytosol of cholesterol and phospholipid in the rat astrocytes treated with apoA-I. and B, translocation of the lipid.
The cytosol fraction was analyzed by immunoblotting for caveolin-1 and cyclophilin A., translocation of cholesterol into the cytosol.
Density gradient analysis of the cytosol lipid of the rat astrocytes.
http://www.jbc.org/cgi/content/full/277/10/7929

  
 Cytosolic Activation of Aromatic and Heterocyclic Amines. Inhibition by Dicoumarol and Enhancement in Viral Hepatitis B
These results suggest that, besides an exclusive cytosolic metabolism, an interaction between microsomal and cytosolic pathways can also occur, such interation being better expressed when the two subfractions are reconstituted in the experimental system used.
The mutagenicity of 1,3-DNP is considerably enhanced only by using the cytosol from uninduced rats, with an NADPH-dependent and dicoumarol-inhibitable mechanism.
Purification of cytosolic DT-diaphorase from control and 3-methylcholanthrene-treated rats.
http://ehp.niehs.nih.gov/members/1994/Suppl-6/deflora-full.html

  
 Apoptotic Cytosol Facilitates Bax Translocation to Mitochondria That Involves Cytosolic Factor Regulated by Bcl-2 -- ...
Aliquots of cytosols (20 µg) were analyzed by Western blotting with anti-Bcl-2 antibody.
that facilitated Bax translocation or that nonapoptotic cytosol
The same experiments were performed as shown in B, and the extent of apoptosis was analyzed by Hoechst 33342 staining.
http://cancerres.aacrjournals.org/cgi/content/full/59/21/5542

  
 Characterization of Caspase Processing and Activation in HL-60 Cell Cytosol Under Cell-free Conditions. NUCLEOTIDE ...
with results obtained using cytosol from rat thymocytes (57).
Previous studies have indicated that this cytochrome c/Apaf-1/caspase-9 pathway can be reconstituted in vitro by incubating
significantly different from those observed when cytosol was analyzed
http://www.jbc.org/cgi/content/full/274/32/22635

  
 Cleaning Oiled Shorelines with a Vegetable Oil Biosolvent
In recent field trials, the CytoSol Process proved effective at removing oil from sensitive marsh plants and mussel beds with much less mortality or injury to the intertidal organisms than has been documented with earlier methods employing hot water, steam, detergents, or mechanical removal of the oiled communities.
A novel shoreline cleanup process has been developed to aid in the removal of crude or fuel oil from shorelines using the highly effective CytoSol "biosolvent" formulation based on vegetable oil methyl esters in combination with bioremediation enhancers.
The CytoSol biosolvent and process are proprietary technologies developed in 1994 as an extension of Dr. von Wedel's experience with designing continuous flow bioreactors and in situ bioremediation technologies for the cleanup of petroleum hydrocarbon contamination in soil and groundwater.
http://www.cytoculture.com/cytosolarticle.htm

  
 Transfer of Free Polymannose-type Oligosaccharides from the Cytosol to Lysosomes in Cultured Human Hepatocellular ...
Inhibition of Cytosolic Processing of Free Polymannose-type Oligosaccharides Affects Their Subcellular Trafficking
within the MBC or are merely associated with the cytosolic face
pathway from the ER into the cytosol and then into an MBC possessing
http://www.jcb.org/cgi/content/full/136/1/45

  
 Visualization of the ER-to-cytosol dislocation reaction of a type I membrane protein -- Fiebiger et al. 21 (5): 1041 -- ...
intermediate(s) of the breakdown reaction are the cytosolic
arise from an unfolded cytosolic EGFP–HC species that
when cytosolically expressed, free EGFP molecules were subjected
http://embojournal.npgjournals.com/cgi/content/full/21/5/1041

  
 Lipid-Assisted Microinjection: Introducing Material into the Cytosol and Membranes of Small Cells -- Laffafian and ...
into the cytosol did not occur immediately on contact between
the technique successfully introduced material into both the cytosol
the micropipette and the cytosol as the concentration of lucifer
http://www.biophysj.org/cgi/content/full/75/5/2558

  
 Identification of 3,5-Diiodo-L-Thyronine-Binding Proteins in Rat Liver Cytosol by Photoaffinity Labeling -- Moreno et ...
During incubation, rat liver cytosol bound a significant proportion
Lennon AM 1992 Purification and characterization of rat brain cytosolic 3, 5, 3'-triiodo-
is still lacking, and the physiological function of cytosolic
http://endo.endojournals.org/cgi/content/full/144/6/2297

  
 Purification and characterization of CTP: cholinephosphate cytidylytransferase from rat liver cytosol.
Purification and characterization of CTP: cholinephosphate cytidylytransferase from rat liver cytosol.
The activity of the L-form is markedly stimulated by liposomes made from rat liver lipids.
The L-form of the cytidylyltransferase was purified from rat liver cytosol.
http://www.arclab.org/medlineupdates/abstract_914832.html

  
 Reduction of Arsenate to Arsenite in Hepatic Cytosol -- Németi and Gregus 70 (1): 4 -- Toxicological Sciences
uracil, or adenine did not affect the cytosolic AsV reduction
most effectively, further assays of cytosolic AsV reductase
Reduction of AsV by rat liver postmitochondrial supernatant (PMSN), microsomes, and cytosol.
http://toxsci.oxfordjournals.org/cgi/content/full/70/1/4

  
 CYTOSOL, Oil Program, US EPA
In practice, the CytoSol would not be emulsified to any great extent during application.
The CytoSol emulsion created microdroplets of product which may have had direct physical effects on the test larvae.
The CytoSol Process is most suitable for the treatment of heavily oiled shorelines that do not respond well to conventional treatments, or that are considered too sensitive for mechanical/pressure wash strategies.
http://www.epa.gov/ceppo/oilspill/ncp/cytosol.htm

  
 Transfer of the Cholera Toxin A1 Polypeptide from the Endoplasmic Reticulum to the Cytosol Is a Rapid Process ...
Transfer of the Cholera Toxin A1 Polypeptide from the Endoplasmic Reticulum to the Cytosol Is a Rapid Process Facilitated by the Endoplasmic Reticulum-Associated Degradation Pathway -- Teter et al.
Transfer of the Cholera Toxin A1 Polypeptide from the Endoplasmic Reticulum to the Cytosol Is a Rapid Process Facilitated by the Endoplasmic Reticulum-Associated Degradation Pathway
Farnesylation of CaaX-tagged diphtheria toxin A-fragment as a measure of transfer to the cytosol.
http://iai.asm.org/cgi/content/full/70/11/6166

  
 Phosphatidylinositol 4,5-bisphosphate stimulates vesicle formation from liposomes by brain cytosol -- Kinuta et al. 99 ...
(A) Western blot analysis of dynamin 1 immunoreactivity of control (Left) and dynamin-depleted (Right) brain cytosol.
Incubation of liposomes with brain cytosol in the
incubated with brain cytosol and nucleotides, and we have developed
http://www.pnas.org/cgi/content/full/99/5/2842

  
 Recognition of bacteria in the cytosol by the immune system
In the new work, Dr. John Brumell and colleagues at The Hospital for Sick Children studied the fate of Salmonella bacteria within the cytosol.
In a finding that broadens our understanding of how the immune system can detect infection, researchers have identified a previously unappreciated way in which bacteria can be recognized inside our cells.
Prior research had shown that bacteria inside the cytosol (the cell's expansive gel-like compartment) could be detected, but how bacteria within the cytosol are recognized has not been clear.
http://www.eurekalert.org/pub_releases/2004-05/cp-rob043004.php

  
 Calbiochem: QIA88: Cytosol/Mitochondria Fractionation Kit
The enriched fractions can be used to study factors of interest using Western blotting, ELISA, or other assays.
Comments: This kit provides a unique combination of reagents useful for the isolation of a highly enriched mitochondrial fraction from the cytosol.
Kit Contents: Mitochondria extraction buffer, cytosol extraction buffer, protease inhibitor cocktail, reducing reagent, and a user protocol.
http://www.emdbiosciences.com/product/QIA88

  
 Cytosol - Wikipedia, the free encyclopedia
Proteins within the cytosol play an important role in signal transduction pathways, glycolysis, and they act as intracellular receptors and form part of the ribosomes, enabling further protein synthesis.
The cytosol (as opposed to cytoplasm, which also includes the organelles) is the internal fluid of the cell, and a large part of cell metabolism occurs here.
This is made of fibrous proteins (microfilaments, microtubules, and intermediate filaments) and (in many organisms) maintains the shape of the cell, anchors organelles, and controls internal movement of structures, e.g., transport vesicles.
http://en.wikipedia.org/wiki/Cytosol

  
 The p67-phox cytosolic peptide of the respiratory burst oxidase from human neutrophils. Functional aspects.
The p67-phox cytosolic peptide of the respiratory burst oxidase from human neutrophils.
Using neutrophils obtained from a patient with this form of the disease, we examined the function of p67-phox in the activation of the oxidase.
Recently, a form of chronic granulomatous disease was described in which the failure of O2- production was associated with the absence of a 67-kD polypeptide (p67-phox) from the cytosol of affected neutrophils.
http://www.pdg.cnb.uam.es/UniPub/iHOP/gp/9619.html

  
 Identification of the Helicobacter pylori VacA Toxin Domain Active in the Cell Cytosol -- de Bernard et al. 66 (12): ...
Cytotoxin production by Campylobacter pylori strains isolated from patients with peptic ulcers and from patients with chronic gastritis only.
be involved in mediating the binding of the toxin to the cytosolic
In this study, we have used cytosolic expression
http://iai.asm.org/cgi/content/full/66/12/6014

  
 Cdc6 protein causes premature entry into S phase in a mammalian cell-free system
phase nuclei where incubated in S phase cytosol (0.6 ng DNA/
phase nuclei initiate only in S phase cytosol whereas G
The same concentration of olomoucine has no effect on the percentages of replicating nuclei when added to control reactions in which S phase 3T3 nuclei are incubated in S phase cytosol (data not shown).
http://www.nature.com/emboj/journal/v17/n24/fig_tab/7591410ft.html

  
 Calcium binding capacity of the cytosol and endoplasmic reticulum of mouse pancreatic acinar cells -- Mogami et al. 518 ...
This was achieved using the droplet technique for measurements of the amount of calcium extruded from cells and simultaneous measurements of the free cytosolic Ca concentration.
The mechanism of buffering was not investigated in this study.
The droplet technique was used in this study to measure total calcium loss from pancreatic acinar cells due to calcium extrusion.
http://jp.physoc.org/cgi/content/full/518/2/463

  
 An unusual sex steroid-binding protein in mature male rat liver cytosol.
Cytosol binding levels of both the [3H]E2 and the [3H]DHT metabolites change in a similar direction in resonse to endocrine manipulation.
The putative liver estrogen receptor level, determined after partial purification (in a redissolved 30% ammonium sulfate-precipitated fraction), seems to change in an opposite direction in response to these same endocrine manipulations.
Mature male rat liver cytosol contains a moderate affinity and capacity estrogen-binding protein in at least a 200-fold higher level than mature female or immature male rat liver cytosol.
http://www.arclab.org/medlineupdates/abstract_107020.html

  
 Photochemical Internalization: A Novel Technology for Delivery of Macromolecules into Cytosol -- Berg, et al. 59 (6): ...
from endosomes and lysosomes into the cytosol was examined.
PCI may have a variety of useful applications for site-specific
III, upon exposure to light, the membranes of the vesicles rupture, and the contents are released into cytosol.
http://cancerres.aacrjournals.org/cgi/content/full/59/6/1180

  
 Two distinct effectors of the small GTPase Rab5 cooperate in endocytic membrane fusion
Black bars correspond to reactions performed in the presence of cytosol treated with preimmune serum, hatched bars to cytosol treated with Rabaptin-5
serum and white bars to cytosol treated with Rabaptin-5 serum.
For the rescue experiments, the fusion reaction in the presence of immunodepleted cytosol for both Rabaptin-5 complexes (lane 6) was supplemented with either 2 or 4
http://www.nature.com/emboj/journal/v17/n7/fig_tab/7590906f6.html

  
 Cell Organelles: Membrane and Cytosol
Though mostly water, the cytosol is full of proteins that control cell metabolism including signal transduction pathways, glycolysis, intracellular receptors, and transcription factors.
s a collective term for the cytosol plus the organelles suspended within the cytosol.
http://www.cellsalive.com/cells/membrane.htm

  
 CYTOSOL LABORATORIES RECEIVES FDA APPROVAL TO MARKET TRICITRASOL® ANTICOAGULANT SODIUM CITRATE CONCENTRATE
The FDA approval of Cytosol Laboratories’ NDA eliminates the need for a doctor to use the “triCitrasol Medical Necessity Qualification Form” to procure the product.
Cytosol has two wholly-owned subsidiaries, Citra Anticoagulants, Inc. and enCyte Systems, Inc. Citra Anticoagulants markets and distributes citrate-based anticoagulants to the medical community.
Since TRICITRASOL was voluntarily recalled in April 2000 for not having appropriate FDA approval, the product has been available for use in granulocytapheresis procedures on a written prescription basis only.
http://www.emediawire.com/releases/2003/7/prweb72710.htm

  
 HW Lecture #22: Glycogen Metabolism
The acyl group is transferred from acyl-CoA to carnitine (catalyzed by carnitine palmitoyl transferase I).
Once inside the mitochondrion, carnitine palmitoyl transferase II catalyzes transfer of the aceyl group from carnitine to Co-A. The product carnitine is returned to the cytosol.
Citrate is then carried into the cytosol via a carrier protein called the Tricarboxylate Transport System.
http://www.uaf.edu/chem/451f00D/HW28.htm

  
 cytosol - definition of cytosol by the Free Online Dictionary, Thesaurus and Encyclopedia.
This information should not be considered complete, up to date, and is not intended to be used in place of a visit, consultation, or advice of a legal, medical, or any other professional.
cytosol - the aqueous part of the cytoplasm within which various particles and organelles are suspended
cytosol - definition of cytosol by the Free Online Dictionary, Thesaurus and Encyclopedia.
http://www.thefreedictionary.com/cytosol

  
 Internal Membranes and Cytosol
(B) The rest of the cell, excluding all these organelles, is called the cytosol, and is the site of many vital cellular activities.
http://www.accessexcellence.org/AB/GG/membranes.html

  
 How to get pure cytosol - BioForum
And at the same time I also need to do caspases assay.
I feel its very hard to get very pure cytosol without any contamination by other non-cytosolic protein, like cytochrome c.
I want get very pure cytosol and to detect this protein being released from organelle under apoptotic stimuli.
http://www.protocol-online.org/forums/index.php?showtopic=2368

  
 Rna1p, the RanGTPase GAP of S. cerevisiae, shuttles between the nucleus and the cytosol.
In all organisms, RanGAP is located primarily in the cytosol, and the GEF is located in the nucleus.
The subcellular distributions led to the prediction that Ran-GTP hydrolysis takes place solely in the cytosol and GDP/GTP exchange solely in the nucleus.
Rna1p, the RanGTPase GAP of S. cerevisiae, shuttles between the nucleus and the cytosol.
http://www.yeastgenome.org/community/meetings/yeast98/abshtml/025.html

  
 The Cytosol
Slightly more than half the cell volume is cytosol (like blood plasma)
http://www.trinity.edu/rblyston/MicroA/Lectures/L01-html/tsld021.htm

  
 Endoplasmic Reticulum Degradation Requires Lumen to Cytosol Signaling: Transmembrane Control of Hrd1p by Hrd3p -- ...
Polyubiquitination Is Required for US11-dependent Movement of MHC Class I Heavy Chain from Endoplasmic Reticulum into Cytosol
to cytosol communication required for regulation of Hrd1p stability
Endoplasmic Reticulum Degradation Requires Lumen to Cytosol Signaling: Transmembrane Control of Hrd1p by Hrd3p
http://www.jcb.org/cgi/content/abstract/151/1/69

  
 Salmon Lab Protocols: Rat Liver Cytosol Prep
Perform steps 1-4 as in "Preparation of Rat Liver Cytosol" above except in step 2, homogenize the minced liver in homogenization buffer containing 0.5 mM MgGTP and 0.25 M sucrose.
In the cold room, collect the supernatant, transfer it to 31.5 ml ultracentrifuge tubes, pair them by weight, and clarify the cytosol by centrifugation at 100,000 x g (29,000 rpm) for 60 min at 4oC in a 50.2Ti rotor.
In the cold room, collect the supernatant (clarified homogenate) and add 2.3 M sucrose stock to make the final concentration 1.25 M sucrose.
http://www.bio.unc.edu/faculty/salmon/lab/protocolsratlivercytosol.html

  
 Respiration
triose-P is converted into hexose-P (in stroma for starch, in cytosol for sucrose)
the relative levels of Pi and triose phosphate in chloroplast vs. cytosol
triose phosphates are transported to the cytosol through a transporter protein
http://www.esf.edu/efb/course/EFB530/Lectures/respirat.htm

  
 Cytosol OEM (Pharmaceutical/Medical Packaging Suppliers Directory)
The company is FDA registered as a medical device and pharmaceutical manufacturer.
Cytosol is CGMP compliant and NSAI ISO 9002/EN46002 certified.
http://www.devicelink.com/company/pmp/co/171/17117.html

  
 LAP3 - Cytosol aminopeptidase
LAP3 is a homologue of At2g24200 (Cytosol aminopeptidase) from Arabidopsis thaliana.
These synonyms are used for gene LAP3 (Cytosol aminopeptidase): EC 3.4.11.5, HGNC:8843, LAP, LAPEP, Leucine aminopeptidase, leucine aminopeptidase 3, Leucyl aminopeptidase, PEPS, Proline aminopeptidase, Prolyl aminopeptidase.
LAP3 is a homologue of Lap3 (Cytosol aminopeptidase) from Mus musculus.
http://www.pdg.cnb.uam.es/UniPub/iHOP/gg/99146.html

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