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| | Apoptosis Protocols and Applications Guide |
 | | Markers of apoptosis such as caspase activity may be expressed only transiently. | | | CN006 Choosing the right cell-based assay for your research | | | Caspase inhibitors have also been used to treat sepsis in mouse models. |
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http://www.promega.com/paguide/chap3.htm
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| | Caspase - Wikipedia, the free encyclopedia |
| | For a good overview of the discovery of not just caspases but other aspects of apoptosis see articles by Danial and Korsmeyer, Yuan and Horvitz, and by Li et al. | | | Failure of apoptosis is one of the main contributions to tumour development and autoimmune diseases; this coupled with the unwanted apoptosis that occurs with ischaemia or Alzheimer's disease, has boomed the interest in caspases as potential therapeutic targets since they were discovered in the mid 1990s. | | | Following this discovery, the other mammalian caspases, in addition to caspases in other organisms such as the fruit fly Drosophila melanogaster, were soon identified and characterised. |
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http://en.wikipedia.org/wiki/Caspase
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| | Caspase Cascade |
| | Caspase independent mechanisms of granzyme B-mediated apoptosis have been suggested. | | | The mitochondrial stress pathway begins with the release of cytochrome c from mitochondria, which then interacts with Apaf-1, causing self-cleavage and activation of caspase-9. | | | The caspases comprise a class of cysteine proteases many members of which are involved in apoptosis. |
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http://www.sigmaaldrich.com/Area_of_Interest/Life_Science/Cell_Signaling/Scientific_Resources/Pathway_Slides___Charts/Caspase_Cascade.html
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| | Apoptosis - BioChemWeb.org |
| | Picture of the Caspase Family - Information on caspases (interleukin-1β-converting enzyme/Ced3-like - ICE-like - family of proteases). | | | Cancer Research UK FACS Laboratory: Apoptosis - Methods for detection of apoptosis using flow cytometry. | | | Apoptosis: Programmed Cell Death - Basic overview covering apoptotic signals, caspases and caspase substrates and pro- and anti-apoptotic proteins. |
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http://www.biochemweb.org/apoptosis.shtml
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| | Flow detection of caspase activity |
| | PhiPhiLux caspase 3-positive cells are also positive for cleaved caspase 3 based on immunolabeling. | | | A variety of fluorogenic caspase substrates have been developed for the detection of functional caspase activity. | | | Caspase 3 activation therefore precedes the morphological changes in apoptotic death; a fraction of morphologically "viable" cells may therefore be preapoptotic. |
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http://home.ncifcrf.gov/ccr/flowcore/caspase3.htm
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| | InterPro: IPR001309 Caspase, p20 subunit |
| | Caspases can have roles other than in apoptosis, such as caspase-1 (interleukin-1 beta convertase) (3.4.22.36), which is involved in the inflammatory process. | | | Caspases are mainly involved in mediating cell death (apoptosis), either as initiators that trigger the cell death process, or as effectors of the process itself [ 2, 3 ]. | | | Caspases may also be involved in cell differentiation [ 4 ]. |
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http://www.ebi.ac.uk/interpro/IEntry?ac=IPR001309
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| | THE ROLE OF CASPASE-3 IN APOPTOSIS |
| | Apoptotic bodies are released from an apoptotic cell. | | | A member of this family, caspase-3 (CPP32, apopain, YAMA) has been identified as being a key mediator of apoptosis of mammalian cells. | | | Kothakota S et al: Caspase 3-generated fragment of gelsolin: effector of morphological change in apoptosis.Science 278, 294-298, 1997 |
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http://www.bioscience.org/news/scientis/caspase.htm
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| | The Caspase Gallery |
| | More than ten caspase family members have been cloned and sequenced by now, and our knowledge of their physiological importance is growing rapidly. | | | This family of cysteine-proteases is a key mediator of apoptosis (programmed cell death), which is a fundamentally important regulatory process to eliminate unwanted cells during development or tissue regulation. | | | The three dimensional structure of two family members (caspase-1/ICE and caspase-3/apopain) has been solved by X-ray diffraction. |
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http://www.enzim.hu/~gaspar/caspase.html
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| | Polyclonal Antibody to Caspase-1 |
| | Caspases are a family of cysteine proteases that are key mediators of programmed cell death or apoptosis (1). | | | All known caspases require cleavage adjacent to aspartates to liberate one large and one small subunit, which associate into a tetramer to form the active enzyme. | | | The active forms of caspases are generated by several stimuli including ligand-receptor interactions, growth factor deprivation and inhibitors of cellular functions. |
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http://www.imgenex.com/antibody_details.php?catalog=IMG-5025
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| | Caspase 7, recombinant human active : CC125 |
| | During apoptosis procaspase-7 is processed at aspartate residues by self-proteolysis and/or cleavage by another caspase. | | | The processed form of caspase-7 consists of large and small subunits which associate to form the active enzyme. | | | The recombinant enzyme can also be used as a positive control in caspase assays or in determining the specificty of caspase substrates.Recommended usage: 0.5-1 unit for fluorometric caspase assays and 2-4 units for colorimetric caspase assays. |
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http://www.chemicon.com/Product/ProductDataSheet.asp?TXTSEARCH=CC119&ProductItem=CC125
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| | Product Detail for Caspase 9 (IM3434) |
| | This antibody crossreacts with mouse and rat caspase 9. | | | This molecule acts as an "initiator" caspase (initiator caspases include caspase 8, 9, and 10) that cleaves "effector" caspases (which include caspase 3, 6, and 7) into active forms that cleave in turn various cellular proteins leading to execution of apoptosis. | | | Caspase 9 (ICE-LAP6 or Mch-6), a 45 kDa protein, is involved in one of the relatively well characterized caspase cascades. |
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http://www.beckman.com/products/instrument/flowcytometry/ecatalog/productDetail.asp?partnumber=IM3434
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| | Caspase Enzymes |
| | 100 units (1 unit is sufficient for one caspase assay reaction). | | | 25 units (1 unit is sufficient for one caspase assay reaction). |
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http://www.gentaur.com/new_page_9.htm
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| | Product Detail for Caspase 7 (IM3433) |
| | This molecule acts as an "effector" caspase (effector caspases inclue caspase 3, 6, and 7) that is cleaved by "initiator" caspases (which include caspase 8, 9, and 10) into active form that cleave in turn various cellular proteins leading to the execution of apoptosis. | | | The 4G2 monoclonal antibody reacts with human caspase 7 in Western blotting and immunoprecipitation. | | | Studies have shown that in Fas-mediated hepatocyte apoptosis, active caspase 7 is associated almost exclusively with mitochondrial and microsomal fractions, whereas active caspase 3 is confined primarily to the cytosol. |
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http://www.beckman.com/products/instrument/flowcytometry/ecatalog/productDetail.asp?partnumber=IM3433&pf=1
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| | Caspases |
| | Nunez, G., Benedict, M.A., Hu, Y., Inohara, N. "Caspases: The proteases of the apoptotic pathway." |
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http://www-personal.umich.edu/~ino/List/31919.htm
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| | Caspase Activation |
| | This is known as the "Caspase Cascade." Caspase-9 also activates caspase-3. | | | Caspase-3 and -9 activate other caspases, cleave enzymes, and activate endonucleases. |
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http://home.fuse.net/apoptosis/caspases.html
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| | [No title] |
| | pAb against Caspase 3, a secondary FITC-labeled goat anti-rabbit abto visualize the bound primary ab | | | pAb against Caspase 3, a secondary PE-labeled goat anti-rabbit ab to visualize the bound primary ab | | | pAb against Caspase 3, secondary FITC-labeled goat anti-rabbit ab to visualize the bound primary ab |
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http://www.stratagene.com/products/showProduct.aspx?source=1&pid=623
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| | Upstate - Product #06-735 - Anti-Caspase 3 ( 06735 ) |
| | Home : Research Products : Caspase : Caspase 3 : | | | Click here to set your country preference and view prices. | | | The antiangiogenic factor 16K PRL induces programmed cell death in endothelial cells by caspase activation. |
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http://www.upstate.com/browse/productdetail.q.ProductID.e.06-735
(170 words)
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| | IST Publications Page |
| | Bistability analyses of a caspase activation model for receptor induced apoptosis. |
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http://www.ist.uni-stuttgart.de/publications
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| | Caspase 8 |
| | Please send any questions or comments about the Human Protein Reference Database to help | | | You are at: Home » Proteins »; Caspase 8 |
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http://www.hprd.org/protein/03459?selectedtab=PTMs
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